Faculdade de Agronomia e Medicina Veterinária., Universidade de Brasília - Campus Darcy Ribeiro, Asa Norte, Brasília, DF, 70910-900, Brazil.
Centro Universitário de Brasília - CEUB 707/907 - Campus Universitário, SEPN - Asa Norte, Brasília, DF, 70790-075, Brazil.
Antonie Van Leeuwenhoek. 2023 Jul;116(7):599-614. doi: 10.1007/s10482-023-01822-3. Epub 2023 May 13.
The usage of microorganisms as biocontrol agents and biofertilizers has been recommended and recognized as an ecologically correct alternative to maintaining the productivity and safety of crops. Thus, the objectives of this work were to characterize twelve strains belonging to Invertebrate Bacteria Collection of Embrapa Genetic Resources and Biotechnology by molecular, morphological, and biochemical methods and to evaluate the pathogenicity of these strains against pests and diseases of agricultural interest. The morphological characteristic of the strains was performed according to the principles of Bergy's Manual of Systematic Bacteriology. The genomes of the 12 strains were sequenced in Macrogen, Inc. (Seoul, Korea) using the HiSeq2000 and GS-FLX Plus high-performance platforms. In the determination of antibiotic sensibility profiles, disc-diffusion methods (Cefar Diagnótica Ltda) were adopted©. Selective bioassays were carried out with insects of the Lepidoptera (Spodoptera frugiperda, Helicoverpa armigera, and Chrysodeixis includens), Coleoptera (Anthonomus grandis), Diptera (Aedes aegypti) and Hemiptera (Euschistus heros) orders, and with the nematode Caenorhabditis elegans. In addition, the antagonistic action of the phytopathogens Fusarium oxysporum f. sp. vasinfectum and Sclerotinia sclerotiorum against the strains under study, and in vitro assays of phosphate solubilization were also performed. Sequencing of the complete genome of the 12 strains determined that all of them belonged to the Bacillus subtilis sensu lato group. In the strains genome were detected genic clusters responsible for encoding secondary metabolites such as surfactin, iturin, fengycins/plipastatin, bacillomycin, bacillisin, and siderophores. Due to the production of these compounds, there was a survival reduction of the Lepidoptera order insects and a reduction in the phytopathogens mycelial growth. These results show that the species of group B. subtilis s.l. can become promising microbiological alternatives to pest and disease control.
利用微生物作为生物防治剂和生物肥料已被推荐和认可为维持作物生产力和安全性的生态正确选择。因此，本工作的目的是通过分子、形态和生化方法对 12 株属于 Embrapa 遗传资源与生物技术的无脊椎细菌收集株进行特征描述，并评估这些菌株对农业害虫和病害的致病性。菌株的形态特征是根据 Bergy 的《系统细菌学手册》的原则进行的。12 株菌的基因组在韩国首尔的 Macrogen, Inc. 公司使用 HiSeq2000 和 GS-FLX Plus 高通量平台进行测序。在抗生素敏感性谱测定中，采用了纸片扩散法（Cefar Diagnótica Ltda）。© 进行了选择性生物测定，使用了鳞翅目（Spodoptera frugiperda、Helicoverpa armigera 和 Chrysodeixis includens）、鞘翅目（Anthonomus grandis）、双翅目（Aedes aegypti）和半翅目（Euschistus heros）昆虫以及线虫 Caenorhabditis elegans。此外，还对抗真菌剂 Fusarium oxysporum f. sp. vasinfectum 和 Sclerotinia sclerotiorum 对研究菌株的拮抗作用以及体外磷酸盐溶解进行了测定。对 12 株菌的全基因组测序表明，它们均属于 Bacillus subtilis sensu lato 组。在这些菌株的基因组中检测到了负责编码表面活性剂、伊枯草菌素、丰原素/plipastatin、杆菌霉素、杆菌肽和铁载体等次生代谢物的基因簇。由于这些化合物的产生，鳞翅目昆虫的存活率降低，植物病原菌的菌丝生长受到抑制。这些结果表明，B. subtilis s.l. 物种可以成为防治害虫和病害的有前途的微生物替代品。
