Department of Biology and Chemistry, College of Sciences, National University of Defense Technology, Changsha 410073, China.
Int J Mol Sci. 2022 Aug 30;23(17):9862. doi: 10.3390/ijms23179862.
Prime editing (PE), as a "search-and-replace" genome editing technology, has shown the attractive potential of versatile genome editing ability, which is, in principle, currently superior to other well-established genome-editing technologies in the all-in-one operation scope. However, essential technological solutions of PE technology, such as the improvement of genome editing efficiency, the inhibition of potential off-targets and intended edits accounting for unexpected side-effects, and the development of effective delivery systems, are necessary to broaden its application. Since the advent of PE, many optimizations have been performed on PE systems to improve their performance, resulting in bright prospects for application in many fields. This review briefly discusses the development of PE technology, including its functional principle, noteworthy barriers restraining its application, current efforts in technical optimization, and its application directions and potential risks. This review may provide a concise and informative insight into the burgeoning field of PE, highlight the exciting prospects for this powerful tool, and provide clues for questions that may propel the field forward.
标题：Prime 编辑技术的发展及其在基因组编辑中的应用
摘要：Prime 编辑（PE）作为一种“搜索和替换”基因组编辑技术，具有多功能基因组编辑能力的吸引力，在某种程度上，其在一次操作中涵盖的范围目前优于其他成熟的基因组编辑技术。然而，PE 技术的一些关键技术解决方案，如提高基因组编辑效率、抑制潜在的脱靶效应和预期编辑导致的意外副作用，以及开发有效的传递系统，对于拓宽其应用范围是必要的。自 PE 问世以来，已经对 PE 系统进行了许多优化，以提高其性能，这为其在许多领域的应用带来了广阔的前景。本文简要讨论了 PE 技术的发展，包括其功能原理、限制其应用的显著障碍、目前在技术优化方面的努力，以及其应用方向和潜在风险。本文可能为快速发展的 PE 领域提供一个简洁而有信息量的视角，突出这一强大工具的令人兴奋的前景，并为可能推动该领域前进的问题提供线索。
