Department of Pharmacognosy, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688, Kraków, Poland.
Department of Food Chemistry and Nutrition, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688, Kraków, Poland.
Chem Biodivers. 2022 Jul;19(7):e202200408. doi: 10.1002/cbdv.202200408. Epub 2022 Jun 2.
In this study, the cytotoxic activity of acetone extracts of Cladonia mitis was assessed with respect to the content of usnic acid, a secondary metabolite commonly present in this species. Following quantitative HPLC analysis of the extracts, usnic acid was isolated by preparative chromatography. The study of cytotoxic activity was performed using the MTT test on three melanoma cell lines - HTB140, A375 and WM793. The selectivity of action was also assessed by comparing the effect towards normal human keratinocytes HaCaT. The results showed a dose-dependent cytotoxic activity of the extracts tested and usnic acid itself, but no relationship was found between the content of usnic acid and the activity of the extracts. Furthermore, the extracts showed varied, but rather low anti-tyrosinase activity. Other in vitro and in vivo studies are necessary to demonstrate that C. mitis extracts may be useful in the adjuvant external treatment of skin melanoma.
在这项研究中，评估了 Cladonia mitis 丙酮提取物的细胞毒性活性，同时考虑了该物种中常见的次级代谢产物——松萝酸的含量。对提取物进行定量 HPLC 分析后，通过制备色谱法分离出松萝酸。使用 MTT 试验在三种黑素瘤细胞系（HTB140、A375 和 WM793）上进行细胞毒性活性研究，并通过比较对正常人类角质形成细胞 HaCaT 的作用来评估其作用的选择性。结果表明，所测试的提取物和松萝酸本身均表现出剂量依赖性的细胞毒性活性，但未发现松萝酸含量与提取物活性之间存在关系。此外，提取物表现出不同但相对较低的酪氨酸酶抑制活性。需要进行其他的体外和体内研究，以证明 C. mitis 提取物可能对皮肤黑素瘤的辅助外部治疗有用。
