Hahm Young Hye, Cho Kun, Ahn Yeong Hee
Rophibio Inc., Osong-eup, Cheongju 28160, Korea.
Center for Research Equipment, Korea Basic Science Institute, Cheongju 28119, Korea.
Plants (Basel). 2021 Oct 30;10(11):2353. doi: 10.3390/plants10112353.
var. (GHL) is one of many herbal plants widely used in hot herbal teas and in oriental prescriptions to treat various diseases. Although the beneficial effects of GHL may be influenced by differences in the composition of active constituents in the herbal extracts, there are few reports on the compositional characteristics of GHL herbal extracts to date. In this study, liquid chromatography-mass spectrometry technology was used for comparative analysis of constituents in hot-water extracts of GHL samples obtained from various cultivating provinces in South Korea. A set of marker panel consisting of nine polyphenolic compounds, including glucuronide conjugates in particular, was constructed and used to monitor the compositional characteristics in each GHL extract. Our findings show that some of the marker compounds, including rosmarinic acid, were persistently observed as major constituents in the analyses of the 22 GHL sample extracts, whereas, interestingly, other marker compounds such as polyphenol-glucuronic acid conjugates displayed dramatic differences in compositional ratios. This chromatographic approach using the marker compound panel can be applied to qualitatively and quantitatively evaluate compositional characteristics in the GHL extracts, and can also be useful for quality assays of the GHL herbal plant in medicinal and industrial fields.
GHL变种是广泛用于热草药茶和东方药方以治疗各种疾病的众多草药之一。尽管GHL的有益效果可能会受到草药提取物中活性成分组成差异的影响，但迄今为止，关于GHL草药提取物组成特征的报道很少。在本研究中，采用液相色谱-质谱技术对从韩国不同种植省份获得的GHL样品热水提取物中的成分进行了比较分析。构建了一组由九种多酚化合物组成的标记物面板，特别是包括葡糖醛酸共轭物，并用于监测每种GHL提取物中的组成特征。我们的研究结果表明，在对22种GHL样品提取物的分析中，一些标记化合物，包括迷迭香酸，一直被观察到是主要成分，而有趣的是，其他标记化合物，如多酚-葡糖醛酸共轭物，在组成比例上表现出显著差异。这种使用标记化合物面板的色谱方法可用于定性和定量评估GHL提取物中的组成特征，也可用于医药和工业领域中GHL草药的质量检测。
