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基于绿色化学利用海洋细菌PBCW2合成金纳米颗粒及其多种活性

Green Chemistry Based Gold Nanoparticles Synthesis Using the Marine Bacterium PBCW2 and Their Multitudinous Activities.

作者信息

Cherian Tijo, Maity Debasis, Rajendra Kumar Ramasamy T, Balasubramani Govindasamy, Ragavendran Chinnasamy, Yalla Suneelkumar, Mohanraju Raju, Peijnenburg Willie J G M

机构信息

Department of Ocean Studies and Marine Biology, Pondicherry University-Port Blair Campus, Port Blair 744 112, Andaman and Nicobar Islands, India.

Aquatic Animal Health and Environment Division, ICAR-Central Institute of Brackishwater Aquaculture, Chennai 600 028, Tamil Nadu, India.

出版信息

Nanomaterials (Basel). 2022 Aug 26;12(17):2940. doi: 10.3390/nano12172940.

Abstract

Green chemistry has paved an 'avant-garde avenue' in the production and fabrication of eco-friendly stable nanoparticles employing the utilization of biological agents. In the present study we present the first report on the potential of the marine bacterium PBCW2 for the extracellular production of gold nanoparticles (AuNPs). Utilizing a variety of methods, AuNPs in the cell-free supernatant of (CFS-LBOE) were identified and their antioxidant, antibacterial, and dye-degrading properties were examined. The visual coloring of the reaction mixture to a ruby red hue showed the production of LBOE-AuNPs; validated by means of XRD, TEM, SEM, XRD, DLS, TGA, and FT-IR analysis. Additionally, the 2,2-diphenyl-1-picrylhydrazyl technique and the well diffusion assay were used to examine their dose-dependent antioxidant and antibacterial activity. These biogenic LBOE-AuNPs showed 91% dye degradation efficiency during catalytic reduction activity on BTB dye, demonstrating their versatility as options for heterogeneous catalysis.

摘要

绿色化学利用生物制剂在环保稳定纳米颗粒的生产和制造方面开辟了一条“先锋大道”。在本研究中,我们首次报道了海洋细菌PBCW2胞外生产金纳米颗粒(AuNPs)的潜力。利用多种方法,对无细胞上清液(CFS-LBOE)中的AuNPs进行了鉴定,并检测了它们的抗氧化、抗菌和染料降解特性。反应混合物呈现出红宝石红色,表明生成了LBOE-AuNPs;通过XRD、TEM、SEM、XRD、DLS、TGA和FT-IR分析进行了验证。此外,采用2,2-二苯基-1-苦基肼技术和琼脂扩散法检测了它们的剂量依赖性抗氧化和抗菌活性。这些生物源LBOE-AuNPs在对BTB染料的催化还原活性中显示出91%的染料降解效率,证明了它们作为多相催化选择的多功能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/942c/9458051/159f44d321c7/nanomaterials-12-02940-g001.jpg

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