亚临界和超临界水提取芦丁的抗氧化和皮肤美白活性。
Antioxidant and Skin Whitening Activities of Sub- and Super-Critical Water Treated Rutin.
机构信息
Department of Food Science and Biotechnology, Chungbuk National University, Cheongju 28644, Korea.
College of Fusion and Convergence, Seowon University, Cheongju 28674, Korea.
出版信息
Molecules. 2022 Aug 25;27(17):5441. doi: 10.3390/molecules27175441.
We focused on the functional components, antioxidant activity, skin-whitening, and anti-wrinkle properties of subcritical and supercritical water (SCW)-treated rutin. Rutin treatments were performed at the following temperature and pressure conditions: 200 °C/15 bar, 300 °C/100 bar, and 400 °C/250 bar. ABTS and DPPH radical scavenging activities and reducing power presented their highest values (1193.72 mg AAE/g, 728.73 mg AAE/g, and 0.65, respectively) at 300 °C/100 bar. The tyrosinase inhibitory activity of SCW-treated rutin was 21.72-60.05% at 1 mg/mL. The ethyl acetate fraction showed 14.91% melanin inhibitory activity at a concentration of 10 µg/mL compared to the α-MSH treatment group. The protein expression inhibition rates of MITF, tyrosinase, TRP-1, and TRP-2 in the ethyl acetate fractions were 14.05%, 72%, 93.05%, and 53.44%, respectively, at a concentration of 10 µg/mL, compared to the control. These results indicate that SCW treatment could be used to develop cosmetic materials and functional food with physiological activity, and that SCW-treated rutin can be used as a skin-whitening cosmetic material.
我们专注于亚临界和超临界水（SCW）处理芦丁的功能成分、抗氧化活性、美白和抗皱特性。芦丁处理在以下温度和压力条件下进行：200°C/15 巴、300°C/100 巴和 400°C/250 巴。ABTS 和 DPPH 自由基清除活性和还原能力在 300°C/100 巴时呈现出最高值（分别为 1193.72 mg AAE/g、728.73 mg AAE/g 和 0.65）。SCW 处理芦丁对酪氨酸酶的抑制活性在 1 mg/mL 时为 21.72%-60.05%。在 10 µg/mL 浓度下，乙酸乙酯馏分与 α-MSH 处理组相比，对黑色素的抑制活性为 14.91%。在 10 µg/mL 浓度下，乙酸乙酯馏分对 MITF、酪氨酸酶、TRP-1 和 TRP-2 的蛋白表达抑制率分别为 14.05%、72%、93.05%和 53.44%。这些结果表明，SCW 处理可用于开发具有生理活性的化妆品材料和功能性食品，并且 SCW 处理的芦丁可用作美白化妆品材料。
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