Bao Xiaojiong, Huang Xiaofei, Jin Xiaoqiang, Hu Qiaoling
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
The Second Affiliated Hospital of Zhejiang University School of Medicine, Zhejiang University, Hangzhou 310009, China.
Polymers (Basel). 2022 Sep 5;14(17):3685. doi: 10.3390/polym14173685.
Bacterial infections occur frequently during the implantation of medical devices, and functional coating is one of the effective means to prevent and remove biofilms. In this study, three different hydrophilic polyoxazolines with carboxyl groups (aPOx: PT1, PT2 and PT3) and bactericidal silver nanoparticles (AgNPs) were synthesized successfully, and an aPOx-AgNP multilayer film was prepared by electrostatic layer-by-layer self-assembly. The effect of charge density and assembly solution concentration was explored, and the optimal self-assembly parameters were established (PT2 1 mg/mL and AgNPs 3 mg/mL). The hydrophilicity of the surface can be enhanced to resist protein adhesion if the outermost layer is aPOx, and AgNPs can be loaded to kill bacteria, thereby realizing the bactericidal anti-adhesion potential integration of the aPOx-AgNP multilayer film. In addition, the aPOx-AgNP multilayer film was found to have the characteristic of intelligent and efficient pH-responsive silver release, which is expected to be used as a targeted anti-biofilm surface of implantable medical devices.
在医疗设备植入过程中细菌感染频繁发生，功能涂层是预防和去除生物膜的有效手段之一。在本研究中，成功合成了三种不同的含羧基亲水性聚恶唑啉（aPOx：PT1、PT2和PT3）和杀菌银纳米颗粒（AgNPs），并通过静电层层自组装制备了aPOx-AgNP多层膜。探索了电荷密度和组装溶液浓度的影响，并确定了最佳自组装参数（PT2 1 mg/mL和AgNPs 3 mg/mL）。如果最外层为aPOx，则可增强表面亲水性以抵抗蛋白质粘附，并且可以负载AgNPs来杀灭细菌，从而实现aPOx-AgNP多层膜的杀菌抗粘附潜在整合。此外，发现aPOx-AgNP多层膜具有智能高效的pH响应性银释放特性，并有望用作可植入医疗设备的靶向抗生物膜表面。
