种复合体中新出现基因型的检测：处理皮肤癣菌生物多样性的建议。
Detection of emerging genotypes in species complex: A proposal for handling biodiversity in dermatophytes.
作者信息
Tang Chao, Ahmed Sarah A, Deng Shuwen, Zhang Lu, Zoll Jan, Al-Hatmi Abdullah M S, Meis Jacques F, Thakur Rameshwari, Kang Yingqian, de Hoog G Sybren
机构信息
The key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou and Key Laboratory of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, China.
Center of Expertise in Mycology, Radboud University Medical Center/Canisius Wilhelmina Hospital, Nijmegen, Netherlands.
出版信息
Front Microbiol. 2022 Aug 23;13:960190. doi: 10.3389/fmicb.2022.960190. eCollection 2022.
A resistant and hypervirulent dermatophyte from India has been described as a taxonomic novelty, , a species of the complex. Rapid detection and correct identification of closely similar dermatophytes with different predilections are essential for efficient clinical management. We evaluated the efficacy of rapid diagnostic methods clinical and environmental strains in the complex. The methods included Real-time-PCR, DermaGenius, LAMP, and MALDI-ToF MS, using rDNA ITS sequences as taxonomic standard. The results show that only MALDI-ToF MS can distinguish 96.97% from other closely related species. The complex comprises numerous clones which may differ in anonymous markers but with similar evolutionary behavior. Therefore, we recommend to distinguish species only when they show an appreciable degree of adaptation and thus are clinically significant. The distinction of remaining clonal diversity is an epidemiological query and can be solved by haplotype numbering.
一种来自印度的具有抗性和高毒力的皮肤癣菌被描述为一个新的分类单元，属于该复合体的一个物种。快速检测和正确鉴定具有不同偏好的相似皮肤癣菌对于有效的临床管理至关重要。我们评估了快速诊断方法对该复合体临床和环境菌株的有效性。这些方法包括实时聚合酶链反应、DermaGenius、环介导等温扩增和基质辅助激光解吸电离飞行时间质谱，使用核糖体DNA内转录间隔区序列作为分类标准。结果表明，只有基质辅助激光解吸电离飞行时间质谱能够将96.97%的该菌与其他密切相关物种区分开来。该复合体包含许多克隆，它们在匿名标记上可能不同，但具有相似的进化行为。因此，我们建议仅在物种表现出明显的适应程度并因此具有临床意义时才进行区分。其余克隆多样性的区分是一个流行病学问题，可以通过单倍型编号来解决。
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