Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
Institute of Mental Health, Tianjin Anding Hospital, Mental Health Center of Tianjin Medical University, Tianjin 300052, P.R. China.
Oncol Rep. 2022 Oct;48(4). doi: 10.3892/or.2022.8400. Epub 2022 Sep 9.
Cell division cycle 45 (CDC45) is an essential protein required for the initiation of DNA replication. In the present study, the role of CDC45 across 33 cancers was systematically investigated. It was observed that the expression of CDC45 was significantly upregulated in most cancers, exhibiting a marked negative correlation with the overall survival. Next, there was no significant difference in prognosis between the genomically altered and unaltered groups with respect to clinical outcomes. A decreased level of CDC45 at the DNA promoter region was also identified in several cancers. Furthermore, CDC45 expression was associated with the levels of tumor‑infiltrating immune cells in some specific cancer types. In addition, CDC45 was associated with mA methylation, and CDC45 expression was primarily positively correlated with 'writers' and 'readers' in various cancers, particularly HNRNPC, RBM15 and YTHDC1. Gene enrichment analysis was also performed. In addition, the AUC of each cancer with respect to its 1‑, 3‑, and 5‑year survival rates were explored. Finally, CCK‑8 assays, EdU assays and cell cycle analysis were conducted. In conclusion, the present study demonstrated that CDC45 may be a potential biomarker and target for cancer treatment.
细胞分裂周期蛋白 45（CDC45）是启动 DNA 复制所必需的一种重要蛋白。在本研究中，系统性地研究了 CDC45 在 33 种癌症中的作用。结果表明，CDC45 的表达在大多数癌症中显著上调，与总生存期呈明显负相关。其次，在临床结局方面，基因组改变组和未改变组之间的预后没有显著差异。在几种癌症中还发现 DNA 启动子区域的 CDC45 水平降低。此外，CDC45 的表达与某些特定癌症类型中肿瘤浸润免疫细胞的水平有关。另外，CDC45 与 mA 甲基化有关，并且在各种癌症中，CDC45 表达主要与“writers”和“readers”呈正相关，尤其是 HNRNPC、RBM15 和 YTHDC1。还进行了基因富集分析。此外，还探讨了每种癌症的 AUC 及其 1、3 和 5 年生存率。最后，进行了 CCK-8 测定、EdU 测定和细胞周期分析。总之，本研究表明，CDC45 可能是癌症治疗的潜在生物标志物和靶标。
