College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
School of Medicine, South China University of Technology, Guangzhou, Guangdong 510006, China.
Int J Biol Macromol. 2022 Nov 30;221:346-354. doi: 10.1016/j.ijbiomac.2022.09.008. Epub 2022 Sep 7.
In this study, Laminaria japonica polysaccharide (LJP) was measured in vitro against three antioxidant indicators: DPPH, ABTS, and hydroxyl. In vivo, LJP investigated thermal tolerance, HO-induced oxidative stress tolerance, and lipofuscin in Caenorhabditis elegans (C. elegans). Following that, after LJP treatment, the effects and underlying mechanisms were investigated at the mRNA and metabolite levels. We discovered the free radical scavenging activity of LJP. The thermal tolerance of C. elegans improved significantly, lowering levels of malondialdehyde, lipofuscin, and reactive oxygen species. Upregulation of Glp-1, Daf-16, Skn-1, and Sod-3 expression and downregulation of Age-1 and Daf-2 expression increased the ability to resist oxidative stress. Metabolomic analysis revealed that LJP promoted alanine, aspartate, and glutamate metabolism, the TCA cycle, butanoate metabolism, and the FOXO signaling pathway expression, resulting in significant changes in (R)-3-hydroxybutyric acid, palmitic acid, L-glutamic acid, L-malic acid, and oleic acid. The present study shows that LJP, as a functional food, has the potential to boost antioxidant capacity and delay aging.
在这项研究中，褐藻多糖（LJP）在体外针对三种抗氧化指标进行了测量：DPPH、ABTS 和羟基。在体内，LJP 研究了热应激、HO 诱导的氧化应激耐受性和秀丽隐杆线虫（C. elegans）中的脂褐素。之后，在 LJP 处理后，在 mRNA 和代谢物水平上研究了其作用和潜在机制。我们发现了 LJP 的自由基清除活性。C. elegans 的热应激耐受性显著提高，降低了丙二醛、脂褐素和活性氧的水平。Glp-1、Daf-16、Skn-1 和 Sod-3 表达上调，Age-1 和 Daf-2 表达下调，增强了其抗氧化应激能力。代谢组学分析显示，LJP 促进了丙氨酸、天冬氨酸和谷氨酸代谢、三羧酸循环、丁酸代谢和 FOXO 信号通路表达，导致（R）-3-羟基丁酸、棕榈酸、L-谷氨酸、L-苹果酸和油酸的含量发生显著变化。本研究表明，LJP 作为一种功能性食品，具有增强抗氧化能力和延缓衰老的潜力。
