Department of Clinical Laboratory, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, China.
Department of Clinical Laboratory, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, China.
J Clin Lab Anal. 2022 Oct;36(10):e24691. doi: 10.1002/jcla.24691. Epub 2022 Sep 9.
DARS2 was overexpressed in multiple tumor types, but the biological role of DARS2 in lung adenocarcinoma (LUAD) have not been elucidated.
Firstly, the DARS2 expression in LUAD was explored using The Cancer Genome Atlas (TCGA). Then, qRT-PCR and Western blot were performed to confirm DARS2 expression in LUAD. Next, Cox regression and Kaplan-Meier methods were utilized to evaluate whether DARS2 expression can affect the overall survival. The relationships between DARS2 expression and clinicopathological characteristics were investigated by TCGA database. Moreover, we utilized Gene Set Enrichment Analysis (GSEA) to detect DARS2-related signaling pathways in LUAD. Finally, the special function of DARS2 in cell proliferation, invasion and apoptosis was assessed in vitro.
The higher expression of DARS2 was found in LUAD compared to para-carcinoma tissues and significantly related to tumor stage, T stage, and M stage. The survival analysis indicated that DARS2 overexpression was related to poor prognosis in LUAD. Multivariate analysis suggested that DARS2 expression was a prognostic indicator. GSEA revealed that DARS2 was primarily involved in cell cycle-related pathways. In addition, upregulation of DARS2 facilitated LUAD cell proliferation, migration, invasion and inhabited apoptosis, DARS2 knockdown showed an opposite result.
DARS2 modulates the proliferation, invasion and apoptosis of LUAD cells, and sever as a promising therapeutic target for LUAD.
DARS2 在多种肿瘤类型中过表达，但 DARS2 在肺腺癌（LUAD）中的生物学作用尚未阐明。
首先，使用癌症基因组图谱（TCGA）探讨 LUAD 中的 DARS2 表达。然后，通过 qRT-PCR 和 Western blot 验证 LUAD 中 DARS2 的表达。接下来，利用 Cox 回归和 Kaplan-Meier 方法评估 DARS2 表达是否能影响总生存期。通过 TCGA 数据库研究 DARS2 表达与临床病理特征之间的关系。此外，我们利用基因集富集分析（GSEA）检测 LUAD 中与 DARS2 相关的信号通路。最后，在体外评估 DARS2 对细胞增殖、侵袭和凋亡的特殊功能。
与癌旁组织相比，LUAD 中 DARS2 的表达更高，且与肿瘤分期、T 分期和 M 分期显著相关。生存分析表明，DARS2 过表达与 LUAD 的不良预后相关。多因素分析提示 DARS2 表达是预后指标。GSEA 表明 DARS2 主要参与细胞周期相关途径。此外，上调 DARS2 促进 LUAD 细胞增殖、迁移、侵袭并抑制细胞凋亡，下调 DARS2 则呈现相反的结果。
DARS2 调节 LUAD 细胞的增殖、侵袭和凋亡，可作为 LUAD 的有前途的治疗靶点。
