Oyabu H, Tabata M, Nakayama F
Dig Dis Sci. 1987 Aug;32(8):809-16. doi: 10.1007/BF01296701.
Bilirubin present in gallstones is mainly in the unconjugated form despite the frequent absence of bacteria in bile. The aim of the present study was to determine if nonbacterial beta-glucuronidase activity and/or nonenzymatic hydrolysis is responsible. Inflammatory cells such as polymorphonuclear leukocytes and lymphocytes appearing with the presence of brown pigment gallstones and inflammation in biliary tract was shown to effect deconjugation of bilirubin conjugates in bile and contribute to their formation in addition to that of bacterial beta-glucuronidase. Gallbladder bile (mean +/- SD, 4.0 +/- 1.6%, N = 29) contained more unconjugated bilirubin than hepatic bile (mean +/- SD, 2.7 +/- 1.1%). In vitro experiments showed the deconjugation to take place during incubation at 37 degrees C without the presence of bacteria. Therefore, transformation of conjugated to unconjugated bilirubin is likely to take place in vivo during the storage in gallbladder, and nonbacterial beta-glucuronidase activity and/or nonenzymatic hydrolysis may be responsible for such transformation.
尽管胆汁中常常不存在细菌，但胆结石中的胆红素主要以未结合形式存在。本研究的目的是确定非细菌性β-葡萄糖醛酸酶活性和/或非酶促水解是否起作用。已表明，随着棕色色素胆结石的出现以及胆道炎症而出现的炎性细胞，如多形核白细胞和淋巴细胞，除了细菌β-葡萄糖醛酸酶外，还会影响胆汁中胆红素结合物的去结合，并促进其形成。胆囊胆汁（平均值±标准差，4.0±1.6%，N = 29）比肝胆汁（平均值±标准差，2.7±1.1%）含有更多的未结合胆红素。体外实验表明，在37℃孵育且无细菌存在的情况下会发生去结合。因此，结合胆红素向未结合胆红素的转化很可能在胆囊储存期间于体内发生，而非细菌性β-葡萄糖醛酸酶活性和/或非酶促水解可能是这种转化的原因。
