耐甲氧西林金黄色葡萄球菌鼻部分离株的抗生素表型和毒素基因的高频性：来自尼日利亚的犬只及其饲养者。

Antibiotypes and high frequency of toxin genes in methicillin-resistant Staphylococcus pseudintermedius from nares of dogs and dog guardians in Nigeria.

机构信息

Department of Applied Microbiology, Faculty of Sciences, Ebonyi State University, P.M.B. 053, Abakaliki, Nigeria; Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo - UNIFESP, Rua Pedro de Toledo, 781, São Paulo SP, Brazil.

Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria.

出版信息

Comp Immunol Microbiol Infect Dis. 2022 Oct;89:101870. doi: 10.1016/j.cimid.2022.101870. Epub 2022 Sep 6.

DOI:10.1016/j.cimid.2022.101870

PMID:36088796

Abstract

To determine the antibiotypes and frequency of toxin genes in methicillin-resistant Staphylococcus pseudintermedius (MRSP), 281 nasal swab samples were collected from dogs and dog guardians in Abakaliki, Southeastern Nigeria. Antimicrobial susceptibility testing was determined by disc diffusion technique while detection of toxin genes was carried out by PCR. Exactly 41 (28.7 %) and 6 (4.3 %) MRSP were obtained from dogs and dog guardians respectively. Isolates exhibited resistance (100-16.7 %) to amoxicillin-clavulanic acid, cephalosporins, fluoroquinolones, and carbapenems. Sec, lukD, siet, and exi toxin genes were harboured by 42 (89.4 %), 47 (100 %), 37 (78.7 %), and 2 (4.3 %) MRSP isolates respectively. This study has shown that dogs and dog guardians in Abakaliki, Southeastern Nigeria are colonized by multiple drug-resistant MRSP which harbour toxin genes. This represents a significant public health problem in veterinary and human medicine.

摘要

为了确定耐甲氧西林中间葡萄球菌（MRSP）的抗生素型和毒素基因的频率，从尼日利亚东南部阿巴卡利基的狗和狗饲养员中采集了 281 份鼻腔拭子样本。采用纸片扩散法进行药敏试验，采用 PCR 法检测毒素基因。分别从狗和狗饲养员中获得了 41 株（28.7%）和 6 株（4.3%）MRSP。分离株对阿莫西林克拉维酸、头孢菌素、氟喹诺酮类和碳青霉烯类表现出耐药性（100-16.7%）。42 株（89.4%）、47 株（100%）、37 株（78.7%）和 2 株（4.3%）MRSP 分离株分别携带 sec、lukD、siet 和 exi 毒素基因。本研究表明，尼日利亚东南部阿巴卡利基的狗和狗饲养员携带产毒素的多药耐药性 MRSP。这在兽医和人类医学中是一个重大的公共卫生问题。