Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.
Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt; Department of Chemistry, College of Science, Sokheer, 32038, Kingdom of Bahrain.
Anal Chim Acta. 2022 Sep 22;1227:340239. doi: 10.1016/j.aca.2022.340239. Epub 2022 Aug 8.
A novel screen-printed electrode (SPE) for potentiometric determination of nalbuphine (NAL) is described. Carboxylated multi-walled carbon nano tubes/polyaniline (f-MWCNTs/PANI) nanocomposite is used as an ion-to- electron transducer and is covered by a selective polyvinyl chloride (PVC) membrane incorporated with molecularly imprinted drug polymer (MIP) beads as a recognition receptor for potentiometric determination a synthetic narcotic, nalbuphine (NAL). The SPE displays a Nernstian response with a slope of 60.3 ± 1.2 mV/decade (R = 0.999) over the concentration range 2.4 × 10 - 5.0 × 10 mol/L and a detection limit of 1.1 × 10 mol/L (0.04 μg/mL) with response time less than 20 s (<20 s). The interfacial capacitance of the proposed SPE is measured using chronopotentiometry (CP) and electrochemical impedance spectroscopy (EIS). The use of the f-MWCNTs/PANI nanocomposite layer improved the interfacial capacitance reached 52.5 μF. Besides, it eliminated the formation of the undesired thin water-layer between the sensing membrane and the conducting substrate. This prevents membrane delamination and increases potential stability. The obtained high selectivity, sensitivity and potential stability offered a great applicability of the proposed SPE for the determination of nalbuphine in hospitals for rapid diagnosis of overdose patients and for quality control/quality assurance in pharmaceutical industry.
一种用于测定纳布啡（NAL）的新型丝网印刷电极（SPE）被描述。羧基多壁碳纳米管/聚苯胺（f-MWCNTs/PANI）纳米复合材料被用作离子-电子转换器，并被一层选择性的聚氯乙烯（PVC）膜覆盖，该膜中嵌入了分子印迹药物聚合物（MIP）珠，作为识别受体，用于测定一种合成麻醉剂纳布啡（NAL）的电化学测定。该 SPE 显示出 Nernstian 响应，斜率为 60.3±1.2 mV/decade（R=0.999），浓度范围为 2.4×10 - 5.0×10 - mol/L，检测限为 1.1×10 - mol/L（0.04μg/mL），响应时间小于 20 s（<20 s）。使用恒电流计时法（CP）和电化学阻抗谱（EIS）测量了所提出的 SPE 的界面电容。使用 f-MWCNTs/PANI 纳米复合材料层提高了界面电容，达到了 52.5 μF。此外，它消除了在传感膜和导电基底之间形成的不需要的薄水层。这防止了膜分层并增加了电势稳定性。所获得的高选择性、灵敏度和电势稳定性为所提出的 SPE 在医院中用于快速诊断过量患者和在制药行业中用于质量控制/质量保证方面测定纳布啡提供了很好的适用性。
