Bogale Achenef, Alemayehu Haile, Nedi Teshome, Engidawork Ephrem
Department of Pharmacy, College of Health Sciences, Debre Tabore University, P.O. Box 272, Debre Tabore, Ethiopia.
Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
Evid Based Complement Alternat Med. 2022 Aug 31;2022:9582687. doi: 10.1155/2022/9582687. eCollection 2022.
is believed to have antidiarrheal potential but with limited scientific evidence. This study aimed investigating antidiarrheal and antibacterial activity of aqueous and 80% methanol seed extracts of the plant in mice and selected diarrhea-causing bacterial strains, respectively.
Castor oil-induced diarrhea, prostaglandin-induced enteropooling, and castor oil-induced charcoal meal test models in mice of either sex using three dose levels (60, 120, and 240 mg/kg) were applied to evaluate antidiarrheal activity. Parameters, including onset, number, wet stool weight, weight and volume of secretion, and intestinal motility, were taken into consideration. The antibacterial activity was assessed on , and using disk diffusion and microdilution techniques.
Compared to controls, pretreatment of mice at the graded dose (60, 120, and 240 mg/kg) resulted in a significant ( < 0.05) drop in frequency of wet stools and watery content of diarrhea as well as in delaying onset of diarrhea. Both extracts exhibited inhibition of diarrhea in a dose-dependent manner in all models used. The extracts also showed significant ( < 0.05) reduction in intestinal motility in castor oil-induced models. Both extracts showed a marginal activity against the selected bacterial strains; a better effect was seen with 80% methanol seed extract.
Both extracts of the plant have beneficial effect in controlling diarrhea. This finding supports the use of the plant as a traditional antidiarrheal remedy.
人们认为其具有止泻潜力，但科学证据有限。本研究旨在分别研究该植物水提取物和80%甲醇种子提取物对小鼠的止泻活性以及对选定的致腹泻细菌菌株的抗菌活性。
采用三种剂量水平（60、120和240mg/kg），对雌雄小鼠进行蓖麻油诱导腹泻、前列腺素诱导肠积液和蓖麻油诱导炭末推进试验模型，以评估止泻活性。考虑的参数包括腹泻发作时间、次数、湿粪重量、分泌液重量和体积以及肠道蠕动。采用纸片扩散法和微量稀释法对[具体细菌名称未给出]、[具体细菌名称未给出]和[具体细菌名称未给出]进行抗菌活性评估。
与对照组相比，按分级剂量（60、120和240mg/kg）对小鼠进行预处理后，湿粪频率和腹泻水样含量显著降低（P<0.05），腹泻发作延迟。在所有使用的模型中，两种提取物均呈剂量依赖性地抑制腹泻。在蓖麻油诱导的模型中，两种提取物还使肠道蠕动显著降低（P<0.05）。两种提取物对选定的细菌菌株均表现出微弱活性；80%甲醇种子提取物的效果更好。
该植物的两种提取物在控制腹泻方面均有有益作用。这一发现支持将该植物用作传统止泻药物。
