Vindel A, Martín-Bourgon C, Saez-Nieto J A
Epidemiol Infect. 1987 Aug;99(1):191-200. doi: 10.1017/s0950268800067029.
A high percentage of non-typable (NT) Staphylococcus aureus strains was isolated in Spanish hospitals during 1984 and 1985. Several alternative methods of typing were employed to study these isolates. These were: phage-typing at 1000 X RTD, phage-typing after heat-treatment (48 degrees C), thermal shock (56 degrees C), reverse-typing and induction of additional phages. Using these methods the number of NT isolates was reduced by 60%. Best results were obtained with heat-treatment. Additional phages and reverse-typing were also useful. A scheme for the study of outbreaks and sporadic cases caused by NT strains is proposed using the methods described.
1984年和1985年期间,西班牙医院分离出了高比例的不可分型(NT)金黄色葡萄球菌菌株。采用了几种替代分型方法来研究这些分离株。这些方法包括:在1000倍常规试验稀释度(RTD)下进行噬菌体分型、热处理(48℃)后进行噬菌体分型、热休克(56℃)、反向分型以及诱导额外噬菌体。使用这些方法,不可分型分离株的数量减少了60%。热处理取得了最佳结果。额外噬菌体和反向分型也很有用。提出了一种使用所述方法研究由不可分型菌株引起的暴发和散发病例的方案。