Castro A J, Tønder N, Sunde N A, Zimmer J
Exp Brain Res. 1987;66(3):533-42. doi: 10.1007/BF00270686.
Fetal cerebral neocortex (E15-17) was grafted into the cortex of newborn (0-1 day old) rats. In some animals the tissue was grafted just caudal to cortical aspiration lesions made immediately prior to grafting. At maturity, transplant efferents were examined by use of the retrogradely transported fluorescent dyes Fast Blue and Diamidino Yellow. Dyes were principally injected in various combinations into the host pyramidal tract decussation and into cervical and lumbar spinal cord levels. In addition some of these animals received injections into the host cortex opposite the transplant, into the ipsilateral thalamus or into the transplant. Transplants were recovered in 26 of 30 animals, and they were typically larger in recipients sustaining aspiration lesions. Histologically, transplants commonly demonstrated laminar patterns that resembled normal cortical supra- and infragranular laminae. Numerous retrogradely-labeled neurons were counted within transplants after injections of fluorescent tracers into the host pyramidal tract decussation or cervical spinal cord. These cells were often located appropriately in areas resembling infragranular layers. Retrogradely labeled neurons were also found within transplants after injections into the host cortex or thalamus. Additionally, numerous host neurons were labeled after Diamidino Yellow injections into the transplant. These findings demonstrate an exchange of connections between host and transplant and suggest the establishment of normal connection patterns.
将胎鼠大脑新皮质（胚胎15 - 17天）移植到新生（0 - 1日龄）大鼠的皮质中。在一些动物中，组织被移植到紧接移植前造成的皮质抽吸损伤部位的尾侧。在成熟时，通过使用逆行运输的荧光染料快蓝和双脒基黄来检查移植体的传出神经。染料主要以各种组合注射到宿主锥体束交叉处以及颈髓和腰髓水平。此外，这些动物中的一些还接受了向移植体对侧的宿主皮质、同侧丘脑或移植体的注射。30只动物中有26只回收了移植体，在遭受抽吸损伤的受体中，移植体通常更大。组织学上，移植体通常表现出类似于正常皮质颗粒上层和颗粒下层的分层模式。在将荧光示踪剂注射到宿主锥体束交叉处或颈髓后，在移植体内计数到大量逆行标记的神经元。这些细胞通常恰当地位于类似于颗粒下层的区域。在向宿主皮质或丘脑注射后，在移植体内也发现了逆行标记的神经元。此外，在向移植体注射双脒基黄后，大量宿主神经元被标记。这些发现证明了宿主与移植体之间连接的交换，并提示建立了正常的连接模式。
