体外毛鞘癌细胞系的形态学和流式细胞术特征分析

Morphological and flow-cytometric characterization of a trichilemmoid carcinoma cell line in vitro.

作者信息

Katsuoka K, Schell H, Hornstein O P, Deinlein E

出版信息

Dermatologica. 1987;175(1):15-22. doi: 10.1159/000248776.

Abstract

A cell line derived from a trichilemmoid carcinoma of a 85-year-old male was established in vitro and maintained in culture for 14 months. From the explant culture to the second passage, the tumour cells propagated slowly. After 6 months (third passage) faster growth behaviour was noticed. In culture the neoplastic cells continued synthesizing abundant glycogen. The DNA histogram of original neoplastic cells revealed a seemingly normal unimodal DNA distribution. The tumour DNA remained apparently stable during the subsequent months in culture. However, after 6 months in culture, flow cytometry disclosed a spontaneous shift in nuclear DNA content. This study enabled us to establish in vitro the malignant properties of the tumour and strongly suggested that it was derived from trichilemmal cells.

摘要

从一名85岁男性的外毛根鞘癌中提取的细胞系在体外建立，并在培养中维持了14个月。从外植体培养到第二代传代，肿瘤细胞增殖缓慢。6个月后（第三代传代），观察到生长行为加快。在培养中，肿瘤细胞持续合成大量糖原。原始肿瘤细胞的DNA直方图显示出看似正常的单峰DNA分布。在随后的培养数月中，肿瘤DNA保持明显稳定。然而，培养6个月后，流式细胞术显示核DNA含量出现自发变化。这项研究使我们能够在体外确定肿瘤的恶性特性，并有力地表明它源自外毛根鞘细胞。