Laboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, Rio Grande do Sul, Brazil.
Unidade Acadêmica de Medicina Veterinária, Universidade Federal de Campina Grande (UFCG), Patos, Paraíba, Brazil.
Braz J Microbiol. 2022 Dec;53(4):2101-2105. doi: 10.1007/s42770-022-00829-8. Epub 2022 Sep 14.
The Adenoviridae family is composed by a high diversity of viruses that are extremely resistant in environment and are frequently excreted in animal reservoir feces for long periods. The knowledge of adenovirus (AdV) diversity among wild species may be important for the understanding of the epidemiology of putative emerging diseases. Cavia aperea aperea, commonly known as wild guinea pigs, wild cavies, or preas, are small herbivorous rodents widely distributed throughout South America and classified in Caviidae family, as well as domestic guinea pigs and capybaras. In order to investigate their potential role as reservoir of zoonotic agents, the present study aimed to verify the presence of AdV in fecal samples of 14 preas from Northeast Brazil. When submitted to nested PCR, two out of 14 samples (14.28%) were positive for AdV and classified as human Mastadenovirus C (HAdV-C) using DNA sequencing and phylogenetic analysis. Wild guinea pigs are synanthropic rodents that live in close contact with humans. The investigation of viral agents in rodents is important due to their potential role as reservoirs of human and animal pathogens. Moreover, the present work presents the first known evidence of HAdV in wild guinea pig stool samples, which may represent both the impact of anthropogenic pollution to wild animals and an important knowledge in terms of human health.
腺病毒科由高度多样化的病毒组成，这些病毒在环境中具有极强的抵抗力，并经常在动物储层粪便中长时间排泄。了解野生动物中的腺病毒（AdV）多样性对于理解潜在新发疾病的流行病学可能很重要。豚鼠，通常被称为野生豚鼠、野生天竺鼠或 preas，是分布在南美洲的小型草食性啮齿动物，分类在豚鼠科中，与家豚鼠和水豚一样。为了调查它们作为人畜共患病原体储存库的潜在作用，本研究旨在检测来自巴西东北部的 14 只 preas 的粪便样本中是否存在 AdV。当进行巢式 PCR 时，14 个样本中有 2 个（14.28%）对 AdV 呈阳性，并通过 DNA 测序和系统发育分析将其分类为人 Mastadenovirus C（HAdV-C）。野生豚鼠是与人类密切接触的栖居性啮齿动物。调查啮齿动物中的病毒因子很重要，因为它们可能是人类和动物病原体的储存库。此外，本研究首次在野生豚鼠粪便样本中发现 HAdV，这可能代表着人为污染对野生动物的影响，以及对人类健康的重要认识。
