Hannover Medical School, Institute of Virology, Hannover, Germany.
German Centre for Infection Research (DZIF), partner site Hannover-, Braunschweig, Germany.
Sci Rep. 2019 Jan 31;9(1):1039. doi: 10.1038/s41598-018-37249-4.
Currently, 88 different Human Adenovirus (HAdV) types are grouped into seven HAdV species A to G. Most types (57) belong to species HAdV-D. Recombination between capsid genes (hexon, penton and fiber) is the main factor contributing to the diversity in species HAdV-D. Noteworthy, species HAdV-C contains so far only five types, although species HAdV-C is highly prevalent and clinically significant in immunosuppressed patients. Therefore, the evolution of species HAdV-C was studied by generating 51 complete genome sequences from circulating strains. Clustering of the whole genome HAdV-C sequences confirmed classical typing results (fifteen HAdV-C1, thirty HAdV-C2, four HAdV-C5, two HAdV-C6). However, two HAdV-C2 strains had a novel penton base sequence and thus were re-labeled as the novel type HAdV-C89. Fiber and early gene region 3 (E3) sequences clustered always with the corresponding prototype sequence but clustering of the E4 region indicated recombination events in 26 out of the 51 sequenced specimens. Recombination of the E1 gene region was detected in 16 circulating strains. As early gene region sequences are not considered in the type definition of HAdVs, evolution of HAdV-C remains on the subtype level. Nonetheless, recombination of the E1 and E4 gene regions may influence the virulence of HAdV-C strains.
目前，有 88 种不同的人类腺病毒（HAdV）被分为 7 个种 A 到 G。大多数类型（57 种）属于种 HAdV-D。衣壳基因（六邻体、五邻体和纤维）之间的重组是导致种 HAdV-D 多样性的主要因素。值得注意的是，种 HAdV-C 迄今为止仅包含 5 种类型，尽管种 HAdV-C 在免疫抑制患者中高度流行且具有重要的临床意义。因此，通过生成来自循环株的 51 个完整基因组序列来研究种 HAdV-C 的进化。全基因组 HAdV-C 序列聚类证实了经典的分型结果（15 种 HAdV-C1、30 种 HAdV-C2、4 种 HAdV-C5、2 种 HAdV-C6）。然而，两种 HAdV-C2 株具有新的五邻体基础序列，因此被重新标记为新型 HAdV-C89。纤维和早期基因区 3（E3）序列总是与相应的原型序列聚类，但 E4 区的聚类表明在 51 个测序样本中有 26 个发生了重组事件。16 个循环株中检测到 E1 基因区的重组。由于早期基因区序列不包含在 HAdV 的分型定义中，因此 HAdV-C 的进化仍处于亚类水平。然而，E1 和 E4 基因区的重组可能会影响 HAdV-C 株的毒力。
