Tang Zizhong, Qin Yihan, Wang Yueyu, Lin Wenjie, Wang Qing, Shen Nayu, Xiao Yirong, Chen Hong, Chen Hui, Bu Tongliang, Li Qingfeng, Yao Huipeng, Feng Shiling, Ding Chunbang
College of Life Sciences, Sichuan Agricultural University, Ya'an, China.
Sichuan Agricultural University Hospital, Sichuan Agricultural University, Ya'an, China.
Front Pharmacol. 2022 Aug 30;13:983716. doi: 10.3389/fphar.2022.983716. eCollection 2022.
The chemical composition and antioxidant activity of extracts (POE) of isolated from Hort have been investigated. However, the biological activity of POE is limited, and its antioxidant, stress resistance and DNA protection effects are unclear. The current study aims to explore the beneficial effects of POE on DNA damage protection in pBR322 plasmid and lymphocytes and stress resistance in . The results showed that POE increased the survival rate of under 35°C, UV and HO stress, attenuated ROS and MDA accumulation, and enhanced the activity of some important enzymes (SOD, CTA, and GSH-PX). In addition, the POE-mediated stress resistance involved the upregulation of the expression of the , , , , , , , , and genes and acted dependently on and rather than . Moreover, POE also reduced lipofuscin levels, but did not prolong the lifespan or damage the growth, reproduction and locomotion of . Furthermore, POE showed a protective effect against DNA scission in the pBR322 plasmid and lymphocytes. These results suggested that extracts have significant anti-stress and DNA protection potential and could be potential drug candidates in the pharmaceutical field, thus greatly broadening the understanding of the biological effects of the endophytic fungus .
已对从霍特分离得到的提取物（POE）的化学成分和抗氧化活性进行了研究。然而，POE的生物活性有限，其抗氧化、抗应激和DNA保护作用尚不清楚。当前研究旨在探讨POE对pBR322质粒和淋巴细胞中DNA损伤保护以及[具体对象]中抗应激的有益作用。结果表明，POE提高了[具体对象]在35°C、紫外线和过氧化氢应激下的存活率, 减轻了活性氧和丙二醛的积累，并增强了一些重要酶（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）的活性。此外，POE介导的抗应激作用涉及[多个基因名称]基因表达的上调，且其作用依赖于[具体基因1]和[具体基因2]而非[具体基因3]。此外，POE还降低了脂褐素水平，但并未延长[具体对象]的寿命或损害其生长、繁殖和运动能力。此外，POE对pBR322质粒和淋巴细胞中的DNA断裂具有保护作用。这些结果表明，[具体对象]提取物具有显著的抗应激和DNA保护潜力，可能成为制药领域的潜在候选药物，从而极大地拓宽了对内生真菌[具体真菌名称]生物学效应的认识。
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