Pichler Alexander, Meinitzer Andreas, Enko Dietmar, Schober Peter, Singer Georg, Castellani Christoph, Herrmann Markus, Holasek Sandra J, Till Holger, Windhaber Jana Maria
Division of General Anesthesiology, Emergency - and Intensive Care Medicine, Medical University of Graz, Graz, Austria.
Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria.
EXCLI J. 2022 Jun 24;21:888-896. doi: 10.17179/excli2022-4889. eCollection 2022.
The maximal oxygen uptake (VOmax) and maximal power output (P) are commonly used parameters to evaluate the endurance fitness status. A connection between exercise and the kynurenine pathway (KP), which describes the metabolism of unused tryptophan, has already been reported. However, a potential association of the KP with endurance fitness levels remains unknown. In this study, adolescent competitive athletes performed an exhaustive incremental exercise test. Blood samples were taken before, directly after, and 30 minutes after the end of exercise. Tryptophan (Trp), kynurenine (Kyn) and kynurenic acid (KA) serum levels were determined by high-performance liquid chromatography (HPLC). Forty-four male and 27 female athletes (median age: 16 years) were recruited. During exhaustive exercise tests, Trp initially declined and then increased 30 minutes after discontinuing exercise. Similar findings were observed for Kyn, whereas KA levels behaved inversely. After incremental exhaustive exercise the relative increase of Trp concentrations, termed the tryptophan-recovery-index (TRI), showed a highly significant positive correlation with VOmax and P (r=0.468 and 0.491, p-values <0.001). There was a significant gender-difference with higher levels of all metabolites at all measured time points in male participants. In the present study, a highly significant correlation was found between the TRI and the maximal oxygen uptake in well-trained athletes. The implementation of TRI can therefore be suggested as a biomarker for physical fitness.
最大摄氧量（VOmax）和最大功率输出（P）是常用于评估耐力健康状况的参数。运动与色氨酸代谢途径（KP）之间的联系已有报道，KP描述了未使用色氨酸的代谢情况。然而，KP与耐力健康水平之间的潜在关联仍不清楚。在本研究中，青少年竞技运动员进行了力竭性递增运动测试。在运动前、运动结束后即刻以及运动结束后30分钟采集血样。采用高效液相色谱法（HPLC）测定血清色氨酸（Trp）、犬尿氨酸（Kyn）和犬尿酸（KA）水平。招募了44名男性和27名女性运动员（中位年龄：16岁）。在力竭性运动测试期间，Trp最初下降，然后在停止运动30分钟后升高。Kyn也观察到类似的结果，而KA水平则相反。在递增力竭性运动后，Trp浓度的相对增加，即色氨酸恢复指数（TRI），与VOmax和P呈高度显著正相关（r = 0.468和0.491，p值<0.001）。在所有测量时间点，男性参与者的所有代谢物水平均较高，存在显著的性别差异。在本研究中，训练有素的运动员的TRI与最大摄氧量之间存在高度显著的相关性。因此，可以建议将TRI作为身体健康的生物标志物。
