Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, 14399-57131, Iran.
J Fluoresc. 2022 Nov;32(6):2297-2307. doi: 10.1007/s10895-022-03023-z. Epub 2022 Sep 16.
Breast cancer is one of the most prevalent cancers and a significant cause of fatalities in women. Diagnosis of breast cancer faces substantial challenges, owing to the multi-factored nature of the illness. Thus, the necessity for an inexpensive, rapid, and non-invasive diagnostic method that enables early detection with high sensitivity and specificity is of primary significance. In this study, a biosensor based on the fluorescence emission of DNA-templated Cu nanoclusters was designed to simultaneously detect and quantify three significant biomarkers of breast cancer (circulating microRNAs, miR-21, miR-195, and miR-155). Fluorescence spectroscopy, FESEM and TEM microscopy, and DLS confirmed the validation of the biosensor. A detection limit of 1.7 pM with a linearity range of 500 nM to 3 µM was obtained. In conclusion, the innovative selection of three biomarkers, the utilization of the HCR process, and an the elaborated design of probes are considered to be among the most important advantages of this biosensor, enabling it a simultaneous triple diagnosis of the blood specific circulating microRNAs without a need for any enzymes, thermo-cycles, expensive or complex facilities, linkers, fluorescent tags, and time-consuming methods.
乳腺癌是最常见的癌症之一，也是女性死亡的主要原因之一。由于该病的多因素性质，乳腺癌的诊断面临着巨大的挑战。因此，需要一种廉价、快速、非侵入性的诊断方法，以便能够早期检测，具有高灵敏度和特异性。在这项研究中，设计了一种基于 DNA 模板 Cu 纳米团簇荧光发射的生物传感器，用于同时检测和定量三种重要的乳腺癌生物标志物（循环 microRNAs、miR-21、miR-195 和 miR-155）。荧光光谱、FESEM 和 TEM 显微镜以及 DLS 证实了生物传感器的验证。获得了 1.7 pM 的检测限和 500 nM 至 3 µM 的线性范围。总之，这种生物传感器的创新选择了三种生物标志物，利用了 HCR 过程，并精心设计了探针，被认为是该生物传感器的最重要优势之一，使其能够同时对血液中特定的循环 microRNAs 进行三重诊断，而无需任何酶、热循环、昂贵或复杂的设备、连接物、荧光标记物以及耗时的方法。
