Wang Shi-Hui, Liang Wen-Xia, Lu Jun, Yao Lu, Wang Juan, Gao Wen-Yuan
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China.
Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China.
Chin Herb Med. 2020 Jul 11;12(3):257-264. doi: 10.1016/j.chmed.2020.02.003. eCollection 2020 Jul.
is a common pathogenic fungus in ginseng cultivation. Both pathogens and antagonistic fungi have been reported to induce plant resistance responses, thereby promoting the accumulation of secondary metabolites. The purpose of this experiment is to compare the advantages of one of the two fungi, in order to screen out more effective elicitors. The mechanism of fungal elicitor-induced plant resistance response is supplemented.
A gradient dilution and the dural culture were carried out to screen strains. The test strain was identified by morphology and 18 s rDNA. The effect of different concentrations (0, 50, 100, 200, 400 mg/L) of sp. YJM-2013 and on fresh weight and ginsenosides accumulation were tested. Signal molecules transduction, expression of transcription factors and functional genes were investigated to study the induction mechanism of fungal elicitors.
Antagonistic fungi of was identified as sp. YJM-2013, which reduced root biomass. The total ginsenosides content of adventitious roots reached the maximum (48.95 ± 0.97 mg/g) treated with sp. YJM-2013 at 200 mg/L, higher than control by 2.59-fold, in which protopanoxadiol-type ginsenosides (PPD) were increased by 4.57 times. Moreover, sp. YJM-2013 activated defense signaling molecules, up-regulated the expression of PgWRKY 1, 2, 3, 5, 7, 9 and functional genes in ginsenosides synthesis.
Compared with the pathogenic fungi , antagonistic fungi sp. YJM-2013 was more conducive to the accumulation of ginsenosides in adventitious roots. sp. YJM-2013 promoted the accumulation of ginsenosides by intensifying the generation of signal molecules, activating the expression of transcription factors and functional genes.
是人参栽培中一种常见的致病真菌。据报道，病原菌和拮抗真菌均可诱导植物抗性反应，从而促进次生代谢产物的积累。本实验的目的是比较这两种真菌中的一种的优势，以便筛选出更有效的诱导子。补充真菌诱导子诱导植物抗性反应的机制。
进行梯度稀释和固体培养以筛选菌株。通过形态学和18 s rDNA对测试菌株进行鉴定。测试了不同浓度（0、50、100、200、400 mg/L）的sp. YJM - 2013和对鲜重和人参皂苷积累的影响。研究信号分子转导、转录因子和功能基因的表达，以探讨真菌诱导子的诱导机制。
的拮抗真菌被鉴定为sp. YJM - 2013，其降低了根生物量。在200 mg/L的sp. YJM - 2013处理下，不定根的总人参皂苷含量达到最高（48.95±0.97 mg/g），比对照高2.59倍，其中原人参二醇型人参皂苷（PPD）增加了4.57倍。此外，sp. YJM - 2013激活了防御信号分子，上调了PgWRKY 1、2、3、5、7、9的表达以及人参皂苷合成中的功能基因。
与致病真菌相比，拮抗真菌sp. YJM - 2013更有利于人参不定根中人参皂苷的积累。sp. YJM - 2013通过增强信号分子的产生、激活转录因子和功能基因的表达来促进人参皂苷的积累。
