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MADS盒基因促进青蒿素生物合成。

MADS-box gene promotes artemisinin biosynthesis in .

作者信息

Chen Tian-Tian, Yao Xing-Hao, Liu Hang, Li Yong-Peng, Qin Wei, Yan Xin, Wang Xiu-Yun, Peng Bo-Wen, Zhang Yao-Jie, Shao Jin, Hu Xin-Yi, Miao Qing, Fu Xue-Qing, Wang Yu-Liang, Li Ling, Tang Ke-Xuan

机构信息

Joint International Research Laboratory of Metabolic and Developmental Sciences, Frontiers Science Center for Transformative Molecules, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

出版信息

Front Plant Sci. 2022 Aug 31;13:982317. doi: 10.3389/fpls.2022.982317. eCollection 2022.

DOI:10.3389/fpls.2022.982317
PMID:36119604
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9473666/
Abstract

The plant is well known for its production of artemisinin, a sesquiterpene lactone that is an effective antimalarial compound. Although remarkable progress has been made toward understanding artemisinin biosynthesis, the effect of MADS-box family transcription factors on artemisinin biosynthesis is still poorly understood. In this study, we identified a MADS transcription factor, AaSEP4, that was predominantly expressed in trichome. AaSEP4 acts as a nuclear-localized transcriptional activator activating the expression of . Dual-luciferase and Yeast one-hybrid assays revealed that AaSEP4 directly bound to the CArG motif in the promoter region of . Overexpression of in significantly induced the expression of and four artemisinin biosynthesis genes, including , , and . Furthermore, the results of high-performance liquid chromatography (HPLC) showed that the artemisinin content was significantly increased in the overexpressed plants. In addition, RT-qPCR results showed that was induced by methyl jasmonic acid (MeJA) treatment. Taken together, these results explicitly demonstrate that AaSEP4 is a positive regulator of artemisinin biosynthesis, which can be used in the development of high-artemisinin yielding varieties.

摘要

该植物因其能产生青蒿素而闻名，青蒿素是一种倍半萜内酯，是一种有效的抗疟化合物。尽管在理解青蒿素生物合成方面已取得显著进展，但MADS-box家族转录因子对青蒿素生物合成的影响仍知之甚少。在本研究中，我们鉴定出一个主要在毛状体中表达的MADS转录因子AaSEP4。AaSEP4作为一种核定位转录激活因子，激活[具体基因名称未给出]的表达。双荧光素酶和酵母单杂交试验表明，AaSEP4直接与[具体基因名称未给出]启动子区域的CArG基序结合。在[具体植物名称未给出]中过表达[具体基因名称未给出]显著诱导了[具体基因名称未给出]以及四个青蒿素生物合成基因（包括[具体基因名称未给出]、[具体基因名称未给出]、[具体基因名称未给出]和[具体基因名称未给出]）的表达。此外，高效液相色谱（HPLC）结果表明，过表达植株中青蒿素含量显著增加。另外，RT-qPCR结果表明，[具体基因名称未给出]受茉莉酸甲酯（MeJA）处理诱导。综上所述，这些结果明确表明AaSEP4是青蒿素生物合成的正调控因子，可用于培育青蒿素高产[具体植物名称未给出]品种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a991/9473666/4c38c1f8c217/fpls-13-982317-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a991/9473666/ff74b7075a81/fpls-13-982317-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a991/9473666/c197f963b67b/fpls-13-982317-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a991/9473666/2ad89460174a/fpls-13-982317-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a991/9473666/de74b2444be0/fpls-13-982317-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a991/9473666/4c38c1f8c217/fpls-13-982317-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a991/9473666/ff74b7075a81/fpls-13-982317-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a991/9473666/c197f963b67b/fpls-13-982317-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a991/9473666/2ad89460174a/fpls-13-982317-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a991/9473666/de74b2444be0/fpls-13-982317-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a991/9473666/4c38c1f8c217/fpls-13-982317-g005.jpg

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The Light- and Jasmonic Acid-Induced Positive Regulates the Initiation of Glandular Secretory Trichome in L.光和茉莉酸诱导的正调控拟南芥腺毛起始的发生。
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