State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, PR China.
State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, PR China.
Food Chem. 2023 Feb 15;402:134165. doi: 10.1016/j.foodchem.2022.134165. Epub 2022 Sep 8.
Ophiopogonis Radix polysaccharides with various bioactivities have caught people's attention in the pharmaceutical and functional food industries. It is necessary to reveal their structures, chain conformations, and solvent behaviors. A neutral polysaccharide named ORP-1 with molecular weight of 3667 Da was obtained from Ophiopogonis Radix. It was composed of d-fructofuranose and d-glucopyranose in the ratio of 0.85:0.15. Methylation, FT-IR and NMR analysis indicated ORP-1 consisted of 2,6-linked-Fruf units as the main chain and 1-linked-Glcp residue at the end. Congo red assay showed ORP-1 had no triple-helix structure. The observation of TEM and AFM found ORP-1 could self-assemble to form colloidal aggregate in water. This phenomenon was verified using CMC determination and MD simulation. Furthermore, intermolecular hydrogen bonds and hydrophobic interactions would be the main forces driving the aggregate. These results provided reference for the study of the chain conformation and behavior of polysaccharides in aqueous solution.
黄精多糖具有多种生物活性，在制药和功能性食品行业引起了人们的关注。揭示其结构、链构象和溶剂行为非常重要。从黄精中提取得到一种中性多糖，命名为 ORP-1，其分子量为 3667 Da，由 0.85:0.15 的 d-果呋喃糖和 d-吡喃葡萄糖组成。甲基化、FT-IR 和 NMR 分析表明，ORP-1 由 2,6-连接的 Fruf 单元作为主链和末端的 1-连接的 Glcp 残基组成。刚果红试验表明 ORP-1 没有三螺旋结构。TEM 和 AFM 的观察发现 ORP-1 可以在水中自组装形成胶体聚集体。这一现象通过 CMC 测定和 MD 模拟得到了验证。此外，分子间氢键和疏水相互作用可能是驱动聚集的主要力。这些结果为研究多糖在水溶液中的链构象和行为提供了参考。
