Zhou Shuxin, Zhu Wei, Qin Xianjin, Li Shipo, Chu Weihua
State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.
School of Pharmaceutical Science, Peking University, Beijing 100089, China.
Antioxidants (Basel). 2022 Aug 26;11(9):1658. doi: 10.3390/antiox11091658.
Fructo-oligosaccharides (FOS) have well-known bifidogenic effects as probiotics. In this study, esterification was adopted for FOS modification to produce better prebiotic properties. We synthesized and characterized acetylated fructo-oligosaccharides (Ac-FOS) and butyrylated fructo-oligosaccharides (Bu-FOS) as candidate prebiotics. Antioxidant activity and prebiotic esactiviti were evaluated as important indicators. We found, surprisingly, that butyrylation was an effective method in significantly improving the antioxidant activity of FOS. The fermentation products of feces from mice added to Ac-FOS and Bu-FOS, were investigated in vitro, including changes of pH values, short-chain fatty acids (SCFAs) production, and microbiota composition. Supplementation of Ac-FOS or Bu-FOS increased pH values and promoted the growth and activity of beneficial intestinal bacteria, such as and . More importantly, the levels of prebiotic SCFAs were obviously elevated as detected by Gas Chromatography-Mass Spectrometry (GC-MS). Results suggest that Ac-FOS and Bu-FOS have great potential applications in SCFA delivery systems and gut microbiota regulation.
低聚果糖（FOS）作为益生菌具有众所周知的双歧杆菌生成作用。在本研究中，采用酯化法对FOS进行改性以产生更好的益生元特性。我们合成并表征了乙酰化低聚果糖（Ac-FOS）和丁酰化低聚果糖（Bu-FOS）作为候选益生元。抗氧化活性和益生元活性被评估为重要指标。令人惊讶的是，我们发现丁酰化是显著提高FOS抗氧化活性的有效方法。对添加了Ac-FOS和Bu-FOS的小鼠粪便发酵产物进行了体外研究，包括pH值变化、短链脂肪酸（SCFA）产生和微生物群组成。添加Ac-FOS或Bu-FOS可提高pH值，并促进有益肠道细菌（如……和……）的生长和活性。更重要的是，通过气相色谱-质谱联用（GC-MS）检测发现，益生元SCFA的水平明显升高。结果表明，Ac-FOS和Bu-FOS在SCFA递送系统和肠道微生物群调节方面具有巨大的潜在应用价值。
