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小鼠c-Ki-ras基因启动子区域的结构与功能特征

Structural and functional characterization of the promoter region of the mouse c-Ki-ras gene.

作者信息

Hoffman E K, Trusko S P, Freeman N, George D L

出版信息

Mol Cell Biol. 1987 Jul;7(7):2592-6. doi: 10.1128/mcb.7.7.2592-2596.1987.

DOI:10.1128/mcb.7.7.2592-2596.1987
PMID:3614201
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365395/
Abstract

We isolated and characterized the 5' region of the mouse c-Ki-ras gene, including a 5' untranslated exon (exon 0). These studies used genetic material from Y1 mouse adrenocortical tumor cells in which the c-Ki-ras gene is amplified and overexpressed. Our data demonstrate that transcription initiates at multiple sites, predicting size heterogeneity at the 5' ends of the c-Ki-ras mRNAs. Using transient expression assays, we identified a genomic fragment within the 5' region which exhibits bidirectional promoter activity.

摘要

我们分离并鉴定了小鼠c-Ki-ras基因的5'区域，包括一个5'非翻译外显子（外显子0）。这些研究使用了来自Y1小鼠肾上腺皮质肿瘤细胞的遗传物质，其中c-Ki-ras基因被扩增并过度表达。我们的数据表明转录起始于多个位点，这预示着c-Ki-ras mRNA的5'端存在大小异质性。通过瞬时表达分析，我们在5'区域内鉴定出一个具有双向启动子活性的基因组片段。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8075/365395/0e8a26ee8b26/molcellb00079-0305-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8075/365395/e90e540f922d/molcellb00079-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8075/365395/d287b04f35f1/molcellb00079-0304-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8075/365395/0e8a26ee8b26/molcellb00079-0305-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8075/365395/e90e540f922d/molcellb00079-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8075/365395/d287b04f35f1/molcellb00079-0304-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8075/365395/0e8a26ee8b26/molcellb00079-0305-a.jpg

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