George D L, Scott A F, de Martinville B, Francke U
Nucleic Acids Res. 1984 Mar 26;12(6):2731-43. doi: 10.1093/nar/12.6.2731.
We have isolated cDNA clones complementary to a c-Ki-ras cellular oncogene that is amplified in Y1 mouse adrenal tumor cells, with the amplified sequences located on double-minute chromatin bodies (DMs) and homogeneously staining chromosomal regions (HSRs). Characterization of the cDNAs included the isolation of corresponding genomic clones, Northern blot analysis of RNA, and DNA sequence analysis. Our studies demonstrate that the c-Ki-ras gene amplified in the Y1 cells is homologous to the human c-Ki-ras2 gene. We have also obtained evidence that, in addition to c-Ki-ras, at least one other transcription unit has been amplified in the mouse adrenal tumor cells. Moreover, by Southern blot analysis of Chinese hamster-mouse somatic cell hybrids, we have determined that the amplified DNA sequences associated with DMs and HSRs, including the c-Ki-ras gene, are present in normal mouse cells on chromosome 6.
我们已经分离出与在Y1小鼠肾上腺肿瘤细胞中扩增的c-Ki-ras细胞癌基因互补的cDNA克隆，扩增序列位于双微小染色质体（DMs）和均匀染色染色体区域（HSRs）上。对这些cDNA的表征包括相应基因组克隆的分离、RNA的Northern印迹分析和DNA序列分析。我们的研究表明，在Y1细胞中扩增的c-Ki-ras基因与人类c-Ki-ras2基因同源。我们还获得了证据，表明除了c-Ki-ras之外，在小鼠肾上腺肿瘤细胞中至少还有一个其他转录单元被扩增。此外，通过对中国仓鼠-小鼠体细胞杂种的Southern印迹分析，我们确定与DMs和HSRs相关的扩增DNA序列，包括c-Ki-ras基因，存在于正常小鼠细胞的6号染色体上。
