National Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Int J Mol Sci. 2022 Sep 7;23(18):10270. doi: 10.3390/ijms231810270.
WRKY transcription factors present unusual research value because of their critical roles in plant physiological processes and stress responses. Rodin (TKS) is a perennial herb of dandelion in the Asteraceae family. However, the research on TKS WRKY TFs is limited. In this study, 72 TKS WRKY TFs were identified and named. Further comparison of the core motifs and the structure of the WRKY motif was analyzed. These TFs were divided into three groups through phylogenetic analysis. Genes in the same group of TkWRKY usually exhibit a similar exon-intron structure and motif composition. In addition, virtually all the TKS WRKY genes contained several -elements related to stress response. Expression profiling of the TkWRKY genes was assessed using transcriptome data sets and Real-Time RT-PCR data in tissues during physiological development, under abiotic stress and hormonal treatments. For instance, the , , and genes were significantly upregulated during cold stress, whereas the gene was upregulated under heat-stress conditions. These results could provide a basis for further studies on the function of the TKS WRKY gene family and genetic amelioration of TKS germplasm.
WRKY 转录因子因其在植物生理过程和应激反应中的关键作用而具有特殊的研究价值。Rodin（TKS）是菊科蒲公英属的一种多年生草本植物。然而，关于 TKS WRKY TFs 的研究有限。本研究鉴定并命名了 72 个 TKS WRKY TFs。进一步比较了核心基序和 WRKY 基序的结构。通过系统发育分析，这些 TF 分为三组。同一组 TkWRKY 的基因通常表现出相似的外显子-内含子结构和基序组成。此外，几乎所有的 TKS WRKY 基因都包含几个与应激反应相关的 - 元件。使用转录组数据集和组织在生理发育过程、非生物胁迫和激素处理下的实时 RT-PCR 数据评估了 TkWRKY 基因的表达谱。例如，基因在冷胁迫下显著上调，而基因在热胁迫条件下上调。这些结果可为进一步研究 TKS WRKY 基因家族的功能和 TKS 种质的遗传改良提供依据。
