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茄子基因家族的鉴定及冷胁迫响应基因的表征

Identification of gene family and characterization of cold stress-responsive genes in eggplant.

作者信息

Yang Yan, Liu Jun, Zhou Xiaohui, Liu Songyu, Zhuang Yong

机构信息

Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China.

出版信息

PeerJ. 2020 Mar 17;8:e8777. doi: 10.7717/peerj.8777. eCollection 2020.

DOI:10.7717/peerj.8777
PMID:32211240
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7083166/
Abstract

BACKGROUND

WRKY proteins play a vital role in the plants response to different stresses, growth and development. Studies of WRKY proteins have been mainly focused on model plant Arabidopsis and a few other vegetable plants. However, the systematical study of eggplant WRKY transcription factor superfamily is scarce.

METHODS

Bioinformatics has been used to identify and characterize the eggplant gene family. For the exploration of the differentially expressed genes, two cultivars with different cold-tolerance were used. Finally, we performed a virus-induced gene silencing (VIGS) experiment to verify the functions of and .

RESULTS

Fifty eight (58) genes encoding eggplant WRKY proteins were identified through searching the eggplant genome. Eggplant WRKY proteins could be classified into three groups or seven subgroups in accordance with other plants. WRKY variants were identified from the eggplant. Gene structure analysis showed that the number of intron in eggplant WRKY family was from 0 to 11, with an average of 4.4. Conserved motif analysis suggested that WRKY DNA-binding domain was conserved in eggplant WRKY proteins. Furthermore, RNA-seq data showed that genes were differentially expressed in eggplant response to cold stress. By using VIGS, the two differentially expressed genes- and were verified in response to cold stress.

DISCUSSIONS

This study provides a foundation for further exploring the functions of WRKY proteins in eggplant response to stresses and eggplant genetic improvement in stresses.

摘要

背景

WRKY蛋白在植物对不同胁迫的响应、生长和发育过程中发挥着至关重要的作用。对WRKY蛋白的研究主要集中在模式植物拟南芥和其他一些蔬菜作物上。然而，关于茄子WRKY转录因子超家族的系统研究却很少。

方法

利用生物信息学方法对茄子WRKY基因家族进行鉴定和特征分析。为了探究差异表达基因，选用了两个耐寒性不同的茄子品种。最后，进行了病毒诱导基因沉默（VIGS）实验来验证两个基因的功能。

结果

通过搜索茄子基因组，共鉴定出58个编码茄子WRKY蛋白的基因。茄子WRKY蛋白可根据其他植物分为3组或7个亚组。从茄子中鉴定出了WRKY变异体。基因结构分析表明，茄子WRKY家族内含子数量为0至11个，平均为4.4个。保守基序分析表明，WRKY DNA结合结构域在茄子WRKY蛋白中是保守的。此外，RNA测序数据显示，多个基因在茄子对冷胁迫的响应中差异表达。通过VIGS验证了两个差异表达基因对冷胁迫的响应。

讨论

本研究为进一步探究WRKY蛋白在茄子胁迫响应中的功能以及茄子胁迫遗传改良提供了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/7174db5efd3b/peerj-08-8777-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/b61b76b38e6e/peerj-08-8777-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/23135a9a693e/peerj-08-8777-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/ab5166166a28/peerj-08-8777-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/c80a419dbc23/peerj-08-8777-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/a2a795fd213c/peerj-08-8777-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/7174db5efd3b/peerj-08-8777-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/b61b76b38e6e/peerj-08-8777-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/23135a9a693e/peerj-08-8777-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/ab5166166a28/peerj-08-8777-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/cea85fa89097/peerj-08-8777-g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/a2a795fd213c/peerj-08-8777-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ff/7083166/7174db5efd3b/peerj-08-8777-g007.jpg

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