Department of Molecular Science and Technology, Ajou University, Suwon 16499, Korea.
Department of Polar Sciences, University of Science and Technology, Incheon 21990, Korea.
Int J Mol Sci. 2022 Sep 17;23(18):10884. doi: 10.3390/ijms231810884.
are widely dispersed in a variety of environments and produce various polysaccharide-degrading enzymes. Here, we report the complete genome of WV33, an agar-degrading bacterium isolated from the stools of Antarctic penguins. The sequenced genome of WV33 represents a single circular chromosome (4,621,116 bp, 35.2% G + C content), containing 3984 coding DNA sequences and 85 RNA-coding genes. The genome of WV33 contains 154 genes that encode carbohydrate-active enzymes (CAZymes). Among the CAZymes, seven putative genes encoding agarases have been identified in the genome. Transcriptional analysis revealed that the expression of these putative agarases was significantly enhanced by the presence of agar in the culture medium, suggesting that these proteins are involved in agar hydrolysis. Pangenome analysis revealed that the genomes of the 27 type strains, including WV33, tend to be very plastic, and strains are unique species with a tiny core genome and a large non-core region. The average nucleotide identity and phylogenomic analysis of the 27 -type strains showed that WV33 was positioned in a unique clade in the evolutionary tree.
它们广泛分布于多种环境中，并能产生各种多糖降解酶。在此，我们报告了一株从南极企鹅粪便中分离到的琼脂降解细菌 WV33 的全基因组序列。 WV33 的测序基因组是一个单一的环形染色体（4621116bp，GC 含量为 35.2%），包含 3984 个编码 DNA 序列和 85 个 RNA 编码基因。 WV33 的基因组包含 154 个编码碳水化合物活性酶（CAZymes）的基因。在 CAZymes 中，在基因组中鉴定出了 7 个可能编码琼脂酶的基因。转录分析显示，这些推测的琼脂酶的表达在培养基中存在琼脂时显著增强，表明这些蛋白参与琼脂水解。泛基因组分析显示，包括 WV33 在内的 27 株模式菌株的基因组具有很强的可塑性，并且 菌株是独特的物种，具有微小的核心基因组和庞大的非核心区域。27 株模式菌株的平均核苷酸同一性和系统基因组分析表明，WV33 在进化树中处于一个独特的分支。
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