Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
National Institute of Biological Resources, 42 Hwangyeong-ro, Seo-gu, Incheon, 22689, Republic of Korea.
Antonie Van Leeuwenhoek. 2023 Oct;116(10):975-986. doi: 10.1007/s10482-023-01858-5. Epub 2023 Aug 5.
In the late 1970s, Flavobacterium bizetiae was first isolated from diseased fish in Canada. After four decades of preservation, it was reported as a novel species in 2020. Here, we report the first complete genome sequence of HJ-32-4, a novel strain of F. bizetiae. Interestingly, HJ-32-4 was isolated from soil in Gangwon-do, Republic of Korea, unlike the other two previously reported F. bizetiae strains which were isolated from fish. We generated a single circular chromosome of HJ-32-4, comprising 5,745,280 bp with a GC content of 34.2%. The average nucleotide identity (ANI) value of 96.2% indicated that HJ-32-4 belongs to F. bizetiae CIP 105534. The virulence factor was not detected in the genome. Comparative genomic analysis of F. bizetiae and major flavobacterial pathogens revealed that F. bizetiae had a larger genome size and the ratio of peptidases (PEP) and glycoside hydrolase (GH) genes of F. bizetiae were lower than those of the rest strains, implying that F. bizetiae exhibits similar characteristics with non-pathogenic strains from a genomic point of view. However, further experimental verification is required to ensure these in silico predictions. This study will provide insight into the overall characteristics of HJ-32-4 compared to other strains.
在 20 世纪 70 年代末，Flavobacterium bizetiae 最初从加拿大患病鱼类中分离出来。经过四十年的保存，2020 年被报道为一个新物种。在这里，我们报告了 HJ-32-4 的第一个完整基因组序列，它是 Flavobacterium bizetiae 的一个新菌株。有趣的是，HJ-32-4 是从韩国江原道的土壤中分离出来的，与其他两种以前报道的从鱼类中分离出来的 Flavobacterium bizetiae 菌株不同。我们生成了 HJ-32-4 的单个圆形染色体，包含 5745280bp，GC 含量为 34.2%。96.2%的平均核苷酸同一性（ANI）值表明 HJ-32-4 属于 Flavobacterium bizetiae CIP 105534。基因组中未检测到毒力因子。Flavobacterium bizetiae 和主要的黄杆菌病原体的比较基因组分析表明，Flavobacterium bizetiae 的基因组大小更大，肽酶（PEP）和糖苷水解酶（GH）基因的比例低于其他菌株，这表明 Flavobacterium bizetiae 在基因组水平上表现出与非致病性菌株相似的特征。然而，需要进一步的实验验证来确保这些计算机预测。这项研究将为 HJ-32-4 与其他菌株的整体特征提供深入了解。
