Phylogeny of Regulators of G-Protein Signaling Genes in and Expression Levels of Three RGSs in Response to Different Terpenoids.

is a bark beetle-vectored pine pathogen in the Chinese white pine beetle () epidemic in Northwest China. colonizes pines despite the trees' massive oleoresin terpenoid defenses. Regulators of G-protein signaling (RGS) proteins modulate heterotrimeric G-protein signaling negatively and play multiple roles in the growth, asexual development, and pathogenicity of fungi. In this study, we have identified three genes, and the phylogenetic analysis shows the highest homology with the regulators of G-protein signaling proteins sequence from and . The expression profiles of three RGSs in the mycelium of treated with six different terpenoids were detected, as well as their growth rates. Under six terpenoid treatments, the growth and reproduction in were significantly inhibited, and the growth inflection day was delayed from 8 days to 12-13 days. By analyzing the expression level of three genes of with different treatments, results indicate that plays a crucial role in controlling fungal growth, and both and are involved in overcoming the host chemical resistances and successful colonization.