State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2023 Aug 21;24(16):13009. doi: 10.3390/ijms241613009.
is a pathogenic fungus of that is epidemic in the Qinling Mountains. However, an effective gene interference strategy is needed to characterize the pathogenic genes in this fungus on a functional level. Using the RNA silencing vector pSilent-1 as a template, we established an RNA interference genetic transformation system mediated by GV3101, which is suitable for the gene study for by homologous recombination and strain interference system screening. The LqFlbA gene was silenced using the RNA interference approach described above, and the resulting transformants displayed various levels of silencing with a gene silencing effectiveness ranging from 41.8% to 91.4%. The LqFlbA-RNAi mutant displayed altered colony morphology, sluggish mycelium growth, and diminished pathogenicity toward the host in comparison to the wild type. The results indicate that this method provides a useful reverse genetic system for studying the gene function of , and that plays a crucial role in the growth, development, and pathogenicity of .
是一种在秦岭南坡流行的致病真菌。然而，需要一种有效的基因干扰策略来在功能水平上对该真菌中的致病基因进行特征描述。我们使用 RNA 沉默载体 pSilent-1 作为模板，建立了一种由 GV3101 介导的 RNA 干扰遗传转化系统，该系统适用于同源重组和菌株干扰系统筛选的基因研究。使用上述 RNA 干扰方法沉默 LqFlbA 基因，得到的转化体显示出不同程度的沉默，基因沉默效率在 41.8%至 91.4%之间。与野生型相比，LqFlbA-RNAi 突变体的菌落形态发生改变，菌丝生长缓慢，对宿主的致病性降低。结果表明，该方法为研究的基因功能提供了一个有用的反向遗传系统，并且在的生长、发育和致病性中起着关键作用。
