首例裂解性/多价噬菌体的发现。
Discovery of the First Lytic / Polyvalent Bacteriophages.
作者信息
Hernandez Santos Haroldo, Clark Justin, Terwilliger Austen, Maresso Anthony
机构信息
Tailored Antibacterials and Innovative Laboratories for Phage (Φ) Research (TAILΦR), Baylor College of Medicine, Houston, Texas, USA.
Molecular Virology and Microbiology Department, Baylor College of Medicine, Houston, Texas, USA.
出版信息
Phage (New Rochelle). 2022 Jun 1;3(2):116-124. doi: 10.1089/phage.2022.0004. Epub 2022 Jun 16.
BACKGROUND
There are no verified lytic phages in the literature and few temperate phage genomes in databases. is an opportunistic zoonotic pathogen of great importance in veterinary and human medicine.
MATERIALS AND METHODS
We discovered phages against canine-derived isolates by screening dog feces, hair, and skin swabs. Fourteen new phages were isolated and characterized by genomic analysis, transmission electron microscopy, and host range determination.
RESULTS
Three phages-DH2, DH5, and DS10, a phage K variant-were predicted lytic by sequencing, a designation supported by mitomycin C induction. All three are and polyvalent phages, with DH2 and DS10 being strong killers of both species.
CONCLUSIONS
We report discovery of the first verified lytic phages and suggest dog hair as a novel reservoir. DH2, DH5, and DS10 are promising candidates toward developing an anti- phage cocktail.
背景
文献中尚无经证实的裂解性噬菌体，数据库中也仅有少数温和噬菌体基因组。[病原体名称]是一种在兽医和人类医学中极为重要的机会性人畜共患病原体。
材料与方法
我们通过筛选犬类粪便、毛发和皮肤拭子，发现了针对犬源[病原体名称]分离株的噬菌体。分离出14种新噬菌体，并通过基因组分析、透射电子显微镜和宿主范围测定对其进行了表征。
结果
通过测序预测有三种噬菌体——DH2、DH5和噬菌体K变体DS10具有裂解性，丝裂霉素C诱导也支持这一认定。这三种噬菌体均为[病原体名称]和[另一病原体名称]多价噬菌体，其中DH2和DS10对这两种病原体都是强力杀手。
结论
我们报告了首次发现经证实的裂解性[病原体名称]噬菌体，并提出犬毛是一种新的噬菌体储存库。DH2、DH5和DS10有望成为开发抗[病原体名称]噬菌体鸡尾酒的候选者。
Zotero 插件