Kim Sang Guen, Giri Sib Sankar, Yun Saekil, Kim Sang Wha, Han Se Jin, Kwon Jun, Oh Woo Teak, Lee Sung Bin, Park Yong Ho, Park Se Chang
Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, South Korea.
Department of Veterinary Microbiology, College of Veterinary Medicine, BK21 Plus Program for Veterinary Science and Research Institute for Veterinary Science, Seoul National University, Seoul, South Korea.
Front Med (Lausanne). 2021 Mar 31;8:524059. doi: 10.3389/fmed.2021.524059. eCollection 2021.
As a primary bacterial pathogen in companion animals, has zoonotic potential. This pathogen exhibits multidrug resistance, including resistance to methicillin, and biofilm-forming ability, making it hard to eradicate with antimicrobial agents. One potential alternative is bacteriophage therapy. In this study, we first characterized the antimicrobial resistance profile of from canine samples and isolated two novel bacteriophages, pSp-J and pSp-S, from canine pet parks in South Korea to potentially control . The biological characteristics of phages were assessed, and the phages could infect most of the methicillin-resistant strains. We found that these phages were stable under the typical environment of the body (~37°C, pH 7). We also assessed bacterial lysis kinetics using the two phages and their cocktail, and found that the phages could prevent biofilm formation at low doses and could degrade biofilm at high doses. Taken together, this study demonstrates that bacteriophages pSp-J and pSp-S isolated in this study can be used to potentially treat methicillin-resistant .
作为伴侣动物中的主要细菌病原体，具有人畜共患病潜力。这种病原体表现出多重耐药性，包括对甲氧西林的耐药性，以及生物膜形成能力，使得用抗菌剂难以根除。一种潜在的替代方法是噬菌体疗法。在本研究中，我们首先对来自犬类样本的抗菌耐药谱进行了表征，并从韩国的犬类宠物公园分离出两种新型噬菌体pSp-J和pSp-S，以潜在地控制。评估了噬菌体的生物学特性，这些噬菌体可以感染大多数耐甲氧西林菌株。我们发现这些噬菌体在身体的典型环境（约37°C，pH 7）下是稳定的。我们还使用这两种噬菌体及其混合物评估了细菌裂解动力学，发现噬菌体在低剂量时可以防止生物膜形成，在高剂量时可以降解生物膜。综上所述，本研究表明，本研究中分离出的噬菌体pSp-J和pSp-S可用于潜在治疗耐甲氧西林。
