Department of Neurosurgery, Affiliated Nanhua Hospital, University of South China, Hengyang, China.
Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Front Immunol. 2022 Sep 8;13:914001. doi: 10.3389/fimmu.2022.914001. eCollection 2022.
Accumulating evidence has demonstrated that the immune cells have an emerging role in controlling anti-tumor immune responses and tumor progression. The comprehensive role of mast cell in glioma has not been illustrated yet. In this study, 1,991 diffuse glioma samples were collected from The Cancer Genome Atlas (TCGA) and the Chinese Glioma Genome Atlas (CGGA). xCell algorithm was employed to define the mast cell-related genes. Based on mast cell-related genes, gliomas were divided into two clusters with distinct clinical and immunological characteristics. The survival probability of cluster 1 was significantly lower than that of cluster 2 in the TCGA dataset, three CGGA datasets, and the Xiangya cohort. Meanwhile, the hypoxic and metabolic pathways were active in cluster 1, which were beneficial to the proliferation of tumor cells. A potent prognostic model based on mast cell was constructed. machine learning, DRG2 was screened out as a characteristic gene, which was demonstrated to predict treatment response and predict survival outcome in the Xiangya cohort. In conclusion, mast cells could be used as a potential effective prognostic factor for gliomas.
越来越多的证据表明，免疫细胞在控制抗肿瘤免疫反应和肿瘤进展方面具有重要作用。然而，肥大细胞在神经胶质瘤中的全面作用尚未阐明。在这项研究中，我们从癌症基因组图谱（TCGA）和中国神经胶质瘤基因组图谱（CGGA）中收集了 1991 例弥漫性神经胶质瘤样本。xCell 算法用于定义肥大细胞相关基因。基于肥大细胞相关基因，将神经胶质瘤分为两个具有明显临床和免疫学特征的聚类。在 TCGA 数据集、三个 CGGA 数据集和湘雅队列中，聚类 1 的生存概率明显低于聚类 2。同时，在聚类 1 中，缺氧和代谢途径是活跃的，这有利于肿瘤细胞的增殖。我们构建了一个基于肥大细胞的强大预后模型。通过机器学习，筛选出 DRG2 作为特征基因，该基因在湘雅队列中可预测治疗反应和生存结果。总之，肥大细胞可以作为神经胶质瘤潜在的有效预后因素。
