Deletion of in a recombinant improved xylose utilization and affected transcription of genes related to amino acid metabolism.

Production of biofuels and biochemicals from xylose using yeast cell factory is of great interest for lignocellulosic biorefinery. Our previous studies revealed that a natural yeast isolate YB-2625 has superior xylose-fermenting ability. Through integrative omics analysis, , which encodes a transcription regulator as well as a subunit of chromatin modifying histone acetyltransferase complexes was revealed to regulate xylose metabolism. Deletion of in YRH396h, which is the haploid version of the recombinant yeast using YB-2625 as the host strain, improved xylose consumption by 28.6%. Comparative transcriptome analysis revealed that deletion down-regulated genes related to mitochondrial function, TCA cycle, ATP biosynthesis, respiration, as well as NADH generation. In addition, the deletion mutant also showed transcriptional changes in amino acid biosynthesis genes. Further analysis of intracellular amino acid content confirmed the effect of on amino acid accumulation during xylose utilization. Our results indicated that is one of the core nodes for coordinated regulation of carbon and nitrogen metabolism in the recombinant This work reveals novel function of Ngg1p in yeast metabolism and provides basis for developing robust yeast strains to produce ethanol and biochemicals using lignocellulosic biomass.