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组织特异性转录组分析揭示了天然反义转录本在热应激反应中的潜在作用。

Tissue-specific transcriptomic analysis uncovers potential roles of natural antisense transcripts in heat stress response.

作者信息

Jin Jingjing, Ohama Naohiko, He Xiujing, Wu Hui-Wen, Chua Nam-Hai

机构信息

China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China.

Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.

出版信息

Front Plant Sci. 2022 Sep 8;13:997967. doi: 10.3389/fpls.2022.997967. eCollection 2022.

DOI:10.3389/fpls.2022.997967
PMID:36160979
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9498583/
Abstract

Natural antisense transcripts (NATs) are an important class of non-coding ribonucleic acids (RNAs) that have been shown to regulate gene expression. Using strand-specific RNA sequencing, 36,317 NAT pairs were identified, and 5,536 were specifically expressed under heat stress. We found distinct expression patterns between vegetative and reproductive tissues for both coding genes and genes encoding NATs. Genes for heat-responsive NATs are associated with relatively high levels of H3K4me3 and low levels of H3K27me2/3. On the other hand, small RNAs are significantly enriched in sequence overlapping regions of NAT pairs, and a large number of heat-responsive NATs pairs serve as potential precursors of nat-siRNAs. Collectively, our results suggest epigenetic modifications and small RNAs play important roles in the regulation of NAT expression, and highlight the potential significance of heat-inducible NATs.

摘要

天然反义转录本（NATs）是一类重要的非编码核糖核酸（RNAs），已被证明可调节基因表达。利用链特异性RNA测序，鉴定出36317对NAT，其中5536对在热胁迫下特异性表达。我们发现，编码基因和编码NAT的基因在营养组织和生殖组织之间具有不同的表达模式。热响应性NAT的基因与相对高水平的H3K4me3和低水平的H3K27me2/3相关。另一方面，小RNAs在NAT对的序列重叠区域显著富集，大量热响应性NAT对作为nat-siRNAs的潜在前体。总体而言，我们的结果表明表观遗传修饰和小RNAs在NAT表达调控中起重要作用，并突出了热诱导NAT的潜在意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/9ba57c1e12cb/fpls-13-997967-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/a613e4a02b91/fpls-13-997967-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/72d9e25edd47/fpls-13-997967-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/423faeb88dbe/fpls-13-997967-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/dbfb1a71103b/fpls-13-997967-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/903d1e2924b4/fpls-13-997967-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/bb35f937e94e/fpls-13-997967-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/9ba57c1e12cb/fpls-13-997967-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/a613e4a02b91/fpls-13-997967-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/72d9e25edd47/fpls-13-997967-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/423faeb88dbe/fpls-13-997967-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/dbfb1a71103b/fpls-13-997967-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/903d1e2924b4/fpls-13-997967-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/bb35f937e94e/fpls-13-997967-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee77/9498583/9ba57c1e12cb/fpls-13-997967-g007.jpg

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