Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, China; Huagongliya (Foshan) Technology Industry Co., Ltd, China.
Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing, China.
Int J Biol Macromol. 2022 Dec 1;222(Pt A):599-609. doi: 10.1016/j.ijbiomac.2022.09.195. Epub 2022 Sep 26.
In the current study, the effects of fermentation manners on the structure and immunomodulatory activity of polysaccharide in longan wine or vinegar were investigated. Compared to longan polysaccharide (CP1), polysaccharide in longan wine (CP2) or vinegar (CP3 and CP4) had smaller molecular weights, and was consisted of more mannose, arabinose, rhamnose, galactose and less glucose. After purification, the major fraction (P1-P4) was obtained from CP1-CP4, respectively. The structures and immunoregulatory activities of P1-P4 were characterized. Fermentation and purification were favorable to increase the immunoregulatory activities of P2-P4, which were contributed to their different structural features. The structure-activity relationship analysis indicated that molecular weight, mannose, rhamnose, glucuronic acid, glucose and arabinose were significantly associated with the cytokines secretion. Compared with other polysaccharides, P3 displayed better immunomodulatory activity due to its lower molecular weight, lower contents of rhamnose and glucose, and higher levels of mannose and arabinose by activating MAPK and PI3K/Akt signaling pathways.
在本研究中，考察了发酵方式对龙眼酒或醋中多糖结构和免疫调节活性的影响。与龙眼多糖（CP1）相比，龙眼酒（CP2）或醋（CP3 和 CP4）中的多糖分子量更小，由更多的甘露糖、阿拉伯糖、鼠李糖、半乳糖和较少的葡萄糖组成。经过纯化，分别从 CP1-CP4 中获得主要级分（P1-P4）。对 P1-P4 的结构和免疫调节活性进行了表征。发酵和纯化有利于提高 P2-P4 的免疫调节活性，这归因于它们不同的结构特征。结构-活性关系分析表明，分子量、甘露糖、鼠李糖、葡萄糖醛酸、葡萄糖和阿拉伯糖与细胞因子分泌显著相关。与其他多糖相比，P3 通过激活 MAPK 和 PI3K/Akt 信号通路，由于其分子量较低、鼠李糖和葡萄糖含量较低、甘露糖和阿拉伯糖含量较高，显示出更好的免疫调节活性。
