检测浸润性导管癌标本中的高危型人乳头瘤病毒 DNA。
Detection of High-Risk Human Papillomavirus DNA in Invasive Ductal Carcinoma Specimens.
机构信息
Cancer Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Asian Pac J Cancer Prev. 2022 Sep 1;23(9):3201-3207. doi: 10.31557/APJCP.2022.23.9.3201.
BACKGROUND
According to several studies, there is an association between human papillomavirus (HPV) and breast cancer. Therefore, detection and genotyping of HPV seem important. The present study aimed to investigate the presence of HPV DNA in breast tissues by analyzing the L1 gene.
MATERIALS AND METHODS
This case-control study was conducted on 63 formalin-fixed paraffin-embedded (FFPE) tissues of invasive ductal carcinoma (IDC) as the case group and 32 FFPE tissues of fibroadenoma as the control group. HPV DNA was detected using the polymerase chain reaction assay. Positive samples were then subjected to genotyping. All statistical analyses were performed in SPSS version 22.0.
RESULTS
The patients' age ranged from 15 to 92 years, with a mean age of 43.54±16.36 years. HPV DNA was detected in 17/95 (17.89%) samples, including 9/32 (28.12%) fibroadenoma samples and 8/63 (12.69%) IDC samples. No significant difference was observed regarding the presence of HPV DNA between the IDC and fibroadenoma tissues (P=0.08). However, a significant difference was found in the detection of high-risk HPV (HR-HPV) between the case and control groups (P=0.03). In the case group, 87.5% of the detected viruses (7/8 samples) were HR-HPV, while in the control group, 22.22% of positive samples (2/9 samples) were HR-HPV (P=0.03). Based on the results, HR-HPV and low-risk HPV genotypes were detected in 53% (9/17) and 47% (8/17) of positive samples, respectively.
CONCLUSION
In this study, 12.69% of IDC samples were positive for HPV genomes, and HR-HPV was detected in 87.5% of these samples. The present results suggest the important role of HR-HPV in the development of breast cancer.
背景
多项研究表明人乳头瘤病毒（HPV）与乳腺癌之间存在关联。因此，HPV 的检测和基因分型似乎很重要。本研究旨在通过分析 L1 基因，检测和分析乳腺组织中 HPV DNA 的存在。
材料和方法
这项病例对照研究共纳入 63 例浸润性导管癌（IDC）福尔马林固定石蜡包埋（FFPE）组织作为病例组，32 例纤维腺瘤 FFPE 组织作为对照组。采用聚合酶链反应（PCR）法检测 HPV DNA。阳性样本进一步进行基因分型。所有统计分析均采用 SPSS 版本 22.0 进行。
结果
患者年龄为 15 岁至 92 岁，平均年龄为 43.54±16.36 岁。在 95 例样本中，有 17 例（17.89%）检测到 HPV DNA，其中 9 例（28.12%）纤维腺瘤样本和 8 例（12.69%）IDC 样本检测到 HPV DNA。IDC 和纤维腺瘤组织中 HPV DNA 的存在无显著差异（P=0.08）。然而，病例组和对照组之间高危型 HPV（HR-HPV）的检测存在显著差异（P=0.03）。在病例组中，检测到的病毒中有 87.5%（7/8 例）为 HR-HPV，而在对照组中，阳性样本中有 22.22%（2/9 例）为 HR-HPV（P=0.03）。基于这些结果，在 17 例阳性样本中，HR-HPV 和低危型 HPV 基因型分别检测到 53%（9/17）和 47%（8/17）。
结论
在本研究中，12.69%的 IDC 样本 HPV 基因组呈阳性，其中 HR-HPV 检出率为 87.5%。本研究结果提示 HR-HPV 在乳腺癌的发生发展中起重要作用。
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