人乳头瘤病毒 DNA 在乳腺癌中的存在：西班牙病例对照研究。
Presence of human papillomavirus DNA in breast cancer: a Spanish case-control study.
机构信息
Department of Obstetrics and Gynecology, University General Hospital of Alicante, c/ Pintor Baeza, 11, 03010, Alicante, Spain.
Department of Genetics, Institute of Cellular and Molecular Studies, Lugo, Spain.
出版信息
BMC Cancer. 2017 May 8;17(1):320. doi: 10.1186/s12885-017-3308-3.
BACKGROUND
Breast cancer is one of the most important neoplasia among women. It was recently suggested that biological agents could be the etiological cause, particularly Human Papilloma Virus (HPV). The aim of this study was to explore the presence of HPV DNA in a case-control study.
METHODS
We performed our study including 251 cases (breast cancer) and 186 controls (benign breast tumors), using three different molecular techniques with PCR (GP5/GP6, CLART® and DIRECT FLOW CHIP®).
RESULTS
HPV DNA was evidenced in 51.8% of the cases and in 26.3% of the controls (p < 0.001). HPV-16 was the most prevalent serotype. The odds ratio (OR) of HPV within a multivariate model, taking into account age and breastfeeding, was 4.034.
CONCLUSIONS
Our study, with methodological rigour and a sample size not previously found in the literature, demonstrate a significant presence of HPV DNA in breast cancer samples. A possible causal relationship, or mediation or not as a cofactor, remains to be established by future studies.
背景
乳腺癌是女性中最重要的肿瘤之一。最近有人提出，生物制剂可能是病因，特别是人乳头瘤病毒（HPV）。本研究旨在通过病例对照研究探讨 HPV DNA 的存在。
方法
我们使用三种不同的分子技术（PCR 中的 GP5/GP6、CLART®和 DIRECT FLOW CHIP®）进行了包括 251 例（乳腺癌）和 186 例对照（良性乳腺肿瘤）的研究。
结果
在 51.8%的病例和 26.3%的对照中检测到 HPV DNA（p<0.001）。HPV-16 是最常见的血清型。在考虑年龄和母乳喂养的多变量模型中，HPV 的比值比（OR）为 4.034。
结论
我们的研究采用了严格的方法学和文献中尚未发现的样本量，证明了 HPV DNA 在乳腺癌样本中的显著存在。未来的研究仍需确定其是否存在因果关系、中介作用或作为共同因素。
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