Obianwuna Uchechukwu Edna, Qiu Kai, Chang Xin-Yu, Zhang Hai-Jun, Wang Jing, Qi Guang-Hai, Sun Tie-Hu, Su Yong-Bo, Wu Shu-Geng
National Engineering Research Center of Biological Feed, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, China.
China Oil Foodstuffs Corporation (COFCO) Nutrition and Health Research Institute, Beijing, China.
Front Microbiol. 2022 Sep 13;13:987241. doi: 10.3389/fmicb.2022.987241. eCollection 2022.
This study focused on evaluating the influence of and strains on egg production, egg quality, immune response and antioxidant function, apparent fecal amino acid digestibility, and jejunal morphology when supplemented as probiotics in the diets of laying hens in the peak phase. A total of 288 healthy 30-week-old Hy-Line Brown laying hens were arbitrarily assigned to four dietary groups, which included control diet and control diet supplemented with 0.02% zlc-17, lwc-13, or zlb-z1, for 84 days. The results showed that dietary and sp. exerted a positively significant influence ( ≤ 0.05) compared to the control group on the performance, egg quality, and physiological response of the birds. The diets could reduce mortality rate and enhance ( ≤ 0.05) egg weight and egg mass, egg production rate, and feed efficiency. Further analysis suggested that the probiotic strains can enhance ( ≤ 0.05) eggshell quality, Haugh unit, thick albumen content, and albumen height. Also, probiotics enhanced ( ≤ 0.05) the antioxidant status increased antioxidant enzymes and jejunal morphology as evidenced by increased villi surface area (VSA), the ratio of villi height to crypt depth, villi width, and villi height, and a significant reduction in crypt depth. Besides, nutrient absorption and retention were enhanced, as apparent fecal amino acid digestibility of key essential amino acids was substantially improved in the diet-based group. The concentrations of immunoglobulin M and A (IgM and IgA) increased significantly ( ≤ 0.05) in the probiotics group and the same effect was notable for complement proteins (C3) and immune organ (Spleen). Conclusively, the supplementation of zlc-17 in comparison to lwc-13 and zlb-z1 strains significantly ( ≤ 0.05) promoted the antioxidant status, modulated the intestinal structure, enhanced amino acid digestibility, and regulated the immunity index of the laying hens, which finally improves the laying performance and egg quality of the laying hens.
本研究着重评估在产蛋高峰期蛋鸡日粮中添加 和 菌株作为益生菌时，对产蛋性能、蛋品质、免疫反应和抗氧化功能、粪便中氨基酸表观消化率以及空肠形态的影响。总共288只健康的30周龄海兰褐蛋鸡被随机分为四个日粮组，包括基础日粮组以及添加0.02% zlc - 17、 lwc - 13或 zlb - z1的基础日粮组，试验为期84天。结果表明，与对照组相比，日粮中的 和 菌株对蛋鸡的生产性能、蛋品质和生理反应产生了显著的正向影响（ ≤ 0.05）。这些日粮可降低死亡率，并提高（ ≤ 0.05）蛋重、产蛋量、产蛋率和饲料效率。进一步分析表明，益生菌菌株可提高（ ≤ 0.05）蛋壳质量、哈夫单位、浓蛋白含量和蛋白高度。此外，益生菌增强了（ ≤ 0.05）抗氧化状态，增加了抗氧化酶活性并改善了空肠形态，表现为绒毛表面积（VSA）、绒毛高度与隐窝深度之比、绒毛宽度和绒毛高度增加，隐窝深度显著降低。此外，营养物质的吸收和保留得到增强，因为在日粮添加组中关键必需氨基酸的粪便表观消化率大幅提高。益生菌组中免疫球蛋白M和A（IgM和IgA）的浓度显著增加（ ≤ 0.05），补体蛋白（C3）和免疫器官（脾脏）也有同样显著的效果。总之，与 lwc - 13和 zlb - z1菌株相比，添加 zlc - 17显著（ ≤ 0.05）促进了蛋鸡的抗氧化状态，调节了肠道结构，提高了氨基酸消化率，并调节了免疫指标，最终改善了蛋鸡的产蛋性能和蛋品质。
