Wang Yajun, Ma Lan, Liu Ziyang, Chen Jingwei, Song Hongxian, Wang Jiajia, Cui Hanwen, Yang Zi, Xiao Sa, Liu Kun, An Lizhe, Chen Shuyan
School of Life Sciences, Lanzhou University, Lanzhou, Gansu, China.
College of Ecology, Lanzhou University, Lanzhou, Gansu, China.
Front Microbiol. 2022 Sep 15;13:984200. doi: 10.3389/fmicb.2022.984200. eCollection 2022.
Plant species and microbial interactions have significant impacts on the diversity of bacterial communities. However, few studies have explored interactions among these factors, such the role of microbial interactions in regulating the effects of plant species on soil bacterial diversity. We assumed that plant species not only affect bacterial community diversity directly, but also influence bacterial community diversity indirectly through changing microbial interactions. Specifically, we collected soil samples associated with three different plant species, one evergreen shrub () and the other two deciduous shrubs ( and ). Soil bacterial community composition and diversity were examined by high-throughput sequencing. Moreover, soil bacterial antagonistic interactions and soil edaphic characteristics were evaluated. We used structural equation modeling (SEM) to disentangle and compare the direct effect of different plant species on soil bacterial community diversity, and their indirect effects through influence on soil edaphic characteristics and microbial antagonistic interactions. The results showed that (1) Plant species effects on soil bacterial diversity were significant; (2) Plant species effects on soil microbial antagonistic interactions were significant; and (3) there was not only a significant direct plant species effect on bacterial diversity, but also a significant indirect effect on bacterial diversity through influence on microbial antagonistic interactions. Our study reveals the difference among plant species in their effects on soil microbial antagonistic interactions and highlights the vital role of microbial interactions on shaping soil microbial community diversity.
植物物种与微生物之间的相互作用对细菌群落的多样性具有重大影响。然而，很少有研究探讨这些因素之间的相互作用，比如微生物相互作用在调节植物物种对土壤细菌多样性影响方面的作用。我们假设植物物种不仅直接影响细菌群落多样性，还通过改变微生物相互作用间接影响细菌群落多样性。具体而言，我们采集了与三种不同植物物种相关的土壤样本，一种是常绿灌木（），另外两种是落叶灌木（和）。通过高通量测序检测土壤细菌群落组成和多样性。此外，还评估了土壤细菌拮抗相互作用和土壤土壤特性。我们使用结构方程模型（SEM）来剖析和比较不同植物物种对土壤细菌群落多样性的直接影响，以及它们通过影响土壤土壤特性和微生物拮抗相互作用产生的间接影响。结果表明：（1）植物物种对土壤细菌多样性的影响显著；（2）植物物种对土壤微生物拮抗相互作用的影响显著；（3）植物物种不仅对细菌多样性有显著的直接影响，还通过影响微生物拮抗相互作用对细菌多样性有显著的间接影响。我们的研究揭示了不同植物物种在对土壤微生物拮抗相互作用影响方面的差异，并突出了微生物相互作用在塑造土壤微生物群落多样性中的关键作用。
