Li Lianbin, Li Kunpeng, Bian Zhengyu, Chen Zeshi, Li Boling, Cui Ke, Wang Fengyang
Key Laboratory of Tropical Animal Breeding and Epidemic Disease Research of Hainan Province, College of Animal Science and Technology, Hainan University, Haikou, Hainan, China.
Hainan Extension Station of Animal Husbandry Technology, Haikou, Hainan, China.
Front Microbiol. 2022 Sep 16;13:951473. doi: 10.3389/fmicb.2022.951473. eCollection 2022.
Gut microbiota plays a critical role in the healthy growth and development of young animals. However, there are few studies on the gut microbiota of young Hainan black goats. In this study, 12 three-month-old weaned lambs with the same birth date were selected and divided into the high body weight group (HW) and low body weight group (LW). The microbial diversity, composition, and predicted function in the feces of HW and LW groups were analyzed by collecting fecal samples and sequencing the 16S rRNA V3-V4 region. The results indicated that the HW group exhibited higher community diversity compared with the LW group, based on the Shannon index. The core phyla of the HW and LW groups were both and . , , and are the core genera of the HW group, and , , and are the core genera of the LW group. In addition, genera such as and , which were positively correlated with body weight, were enriched in the HW group; those genera, such as and , which were negatively correlated with body weight, were enriched in the LW group. Differential analysis of the KEGG pathway showed that Amino Acid Metabolism, Energy Metabolism, Carbohydrate Metabolism, and Nucleotide Metabolism were enriched in the HW group, while Cellular Processes and Signaling, Lipid Metabolism, and Glycan Biosynthesis and Metabolism were enriched in the LW group. The results of this study revealed the gut microbial characteristics of Hainan black goats with different body weights at weaning age and identified the dominant flora that contributed to their growth.
肠道微生物群对幼龄动物的健康生长和发育起着关键作用。然而，关于海南黑山羊幼崽肠道微生物群的研究较少。在本研究中，选取了12只出生日期相同的3月龄断奶羔羊，分为高体重组（HW）和低体重组（LW）。通过采集粪便样本并对16S rRNA V3-V4区域进行测序，分析了HW组和LW组粪便中的微生物多样性、组成及预测功能。结果表明，基于香农指数，HW组的群落多样性高于LW组。HW组和LW组的核心菌门均为 和 。 、 和 是HW组的核心属， 、 和 是LW组的核心属。此外，与体重呈正相关的 和 等属在HW组中富集；与体重呈负相关的 和 等属在LW组中富集。KEGG通路差异分析表明，HW组中氨基酸代谢、能量代谢、碳水化合物代谢和核苷酸代谢富集，而LW组中细胞过程与信号传导、脂质代谢以及聚糖生物合成与代谢富集。本研究结果揭示了断奶期不同体重海南黑山羊的肠道微生物特征，并确定了对其生长有贡献的优势菌群。
