School of Economics and Management, Qiannan Normal College for Nationalities, 558000, China.
Biomed Res Int. 2022 Sep 23;2022:9978571. doi: 10.1155/2022/9978571. eCollection 2022.
CRISPR/Cas9 technology has become the most examined gene editing technology in recent years due to its simple design, yet low cost, high efficiency, and simple operation, which can also achieve simultaneous editing of multiple loci. It can also be carried out without using plasmids, saving lots of troubles caused by plasmids. CRISPR/Cas9 has shown great potential in the study of genes or genomic functions in microorganisms, plants, animals, and human beings. In this review, we will examine the history, structure, and basic mechanisms of the CRISPR/Cas9 system, describe its great value in precision medicine and sgRNA library screening, and dig its great potential in a new field: DNA information storage.
CRISPR/Cas9 技术因其设计简单、成本低、效率高、操作简单，同时可以实现多个基因座的同时编辑，而且不需要质粒，避免了质粒带来的诸多麻烦，成为近年来研究最多的基因编辑技术。CRISPR/Cas9 在微生物、植物、动物和人类的基因或基因组功能研究中显示出巨大的潜力。本文综述了 CRISPR/Cas9 系统的历史、结构和基本机制，描述了其在精准医学和 sgRNA 文库筛选中的重要价值，并挖掘了其在 DNA 信息存储这一新兴领域的巨大潜力。
