利用 ISSR 和 SRAP 标记分析中国 菌株的遗传多样性。
Analysis of genetic diversity among Chinese strains using ISSR and SRAP markers.
机构信息
Jiangxi Agricultural University, Bioengineering and Technological Research Centre for Edible and Medicinal Fungi, Nanchang, China.
Jiangxi Agricultural University, College of Bioscience and Bioengineering, Nanchang, China.
出版信息
PeerJ. 2022 Sep 29;10:e14037. doi: 10.7717/peerj.14037. eCollection 2022.
BACKGROUND
is an edible and medicinal fungal species commonly cultivated in China. The major problems currently facing by growers of is the random labeling of strains and strains aging and degeneration. Therefore, an evaluation of genetic diversity is essential for the conservation and reproducing programs of this species.
METHODS
In the present study, 24 widely cultivated strains were collected from the main producing areas of China, and the genetic diversity analysis was performed. DNA polymorphism among these Chinese strains was analyzed using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers.
RESULTS
Eight ISSR primers amplified a total of 75 DNA fragments of which 61 (81.33%) were polymorphic. Fifteen SRAP primer combinations amplified 166 fragments of which 132 (79.52%) were polymorphic. Cluster analysis showed that the strains fall into five groups with a genetic distance values ranging from 0.06 to 0.60 by ISSR analysis, while the SRAP analysis divided the test strains into four groups within the range of genetic distance from 0.03 to 0.57. The results of the present study reveal a high level of genetic diversity among the widely cultivated strains.
背景
是一种可食用和药用的真菌物种，在中国广泛种植。目前，种植者面临的主要问题是菌株的随意标记以及菌株的老化和退化。因此，对该物种进行遗传多样性评估对于其保护和繁殖计划至关重要。
方法
本研究从中国主要产区收集了 24 株广泛种植的菌株，并进行了遗传多样性分析。利用 ISSR 和 SRAP 标记分析了这些中国 菌株的 DNA 多态性。
结果
8 条 ISSR 引物共扩增出 75 条 DNA 片段，其中 61 条（81.33%）为多态性。15 条 SRAP 引物组合共扩增出 166 条片段，其中 132 条（79.52%）为多态性。聚类分析显示，通过 ISSR 分析，菌株分为 5 组，遗传距离值在 0.06 到 0.60 之间，而 SRAP 分析将测试菌株分为遗传距离在 0.03 到 0.57 之间的 4 组。本研究结果表明，广泛种植的 菌株具有较高的遗传多样性。
Zotero 插件