Tai Bui Huu, Yen Pham Hai, Hoang Nguyen Huy, Thanh Huong Phan Thi, Dung Nguyen Viet, Van Thanh Bui, Cuong Nguyen The, Bang Ngo Anh, Nhiem Nguyen Xuan, Van Kiem Phan
Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST) 18 Hoang Quoc Viet, Cau Giay Hanoi Vietnam
Graduate University of Science and Technology, VAST 18 Hoang Quoc Viet, Cau Giay Hanoi Vietnam.
RSC Adv. 2022 Sep 7;12(39):25433-25439. doi: 10.1039/d2ra05052h. eCollection 2022 Sep 5.
Five new dibenzocyclooctadiene lignans, named kadsuindutains A-E (1-5), and three known ones schizanrin F (6), schizanrin O (7), and schisantherin J (8) were isolated from the stems of . Their structures were determined by analyses of HR-ESI-MS, NMR, and ECD spectra. Compounds 1-5 contain a 2',4'-dioxygenated-2',3'-dimethylbutyryl moiety which is rarely reported for dibenzocyclooctadiene lignans. Molecular docking predicted that compounds 1-8 displayed good binding affinity to the active site of iNOS and TNF-α proteins but unstable binding to the active site of COX-2 protein. Additionally, experiments showed that compounds 1-8 inhibited NO production in LPS-activated RAW264.7 cells with IC values from 10.7 μM to 34.0 μM, compared to the positive control L-NMMA (IC = 31.2 μM).
从……的茎中分离出了五种新的二苯并环辛二烯木脂素，命名为卡苏因杜坦 A-E（1-5），以及三种已知的木脂素，裂环马兜铃素 F（6）、裂环马兜铃素 O（7）和南五味子素 J（8）。通过高分辨电喷雾电离质谱（HR-ESI-MS）、核磁共振（NMR）和电子圆二色光谱（ECD）分析确定了它们的结构。化合物 1-5 含有一个 2',4'-二氧代-2',3'-二甲基丁酰基部分，这在二苯并环辛二烯木脂素中很少见。分子对接预测化合物 1-8 对诱导型一氧化氮合酶（iNOS）和肿瘤坏死因子-α（TNF-α）蛋白的活性位点表现出良好的结合亲和力，但对环氧合酶-2（COX-2）蛋白的活性位点结合不稳定。此外，实验表明化合物 1-8 在脂多糖（LPS）激活的 RAW264.7 细胞中抑制一氧化氮（NO）的产生，其半数抑制浓度（IC）值在 10.7 μM 至 34.0 μM 之间，与阳性对照 L-硝基精氨酸甲酯（L-NMMA，IC = 31.2 μM）相比。
