School of Chemical Engineering, Hanoi University of Science and Technology, Hanoi, 100000, Vietnam.
Intellectual Property Office of Vietnam, Hanoi, 100000, Vietnam.
Chem Biodivers. 2023 Sep;20(9):e202300904. doi: 10.1002/cbdv.202300904. Epub 2023 Sep 8.
A chemical investigation of K. heteroclite led to isolation of two new dibenzocyclooctadienes (1 and 2) together with 14 known compounds (3-16) by using multiple chromatographic techniques. New compounds (1 and 2) were obtained and identified by spectroscopic methods (HR-ESI-MS, 1D and 2D NMR, and ECD) as well as by comparison of their experimental data with those reported in the literatures. All the isolates were evaluated for their ability to modulate TNF-α production in lipopolysaccharide (LPS) stimulated RAW264.7 cells. Among them, compound 5 displayed the most inhibition against tumor necrosis factor (TNF)-α production with IC value of 6.16±0.14 μM. Whereas, compounds (1, 3, and 6) showed the significant inhibition (IC values ranging from 9.41 to 14.54 μM), and compounds (2, 4, 9, 10, 13, 15, and 16) exhibited moderate inhibition (IC values ranging from 19.27 to 40.64 μM) toward TNF-α production, respectively.
对 K. heteroclite 的化学研究通过多种色谱技术分离得到了两个新的二苯并环辛二烯（1 和 2）以及 14 个已知化合物（3-16）。新化合物（1 和 2）通过光谱方法（高分辨电喷雾电离质谱、1D 和 2D NMR 和 ECD）以及与其文献报道的实验数据进行比较来获得和鉴定。所有分离物均评估了它们在脂多糖（LPS）刺激的 RAW264.7 细胞中调节 TNF-α 产生的能力。其中，化合物 5 对肿瘤坏死因子（TNF）-α产生的抑制作用最强，IC 值为 6.16±0.14 μM。而化合物（1、3 和 6）显示出显著的抑制作用（IC 值范围为 9.41 至 14.54 μM），化合物（2、4、9、10、13、15 和 16）对 TNF-α 的产生具有中等抑制作用（IC 值范围为 19.27 至 40.64 μM）。
